17r hdha

Manufactured by Cayman Chemical

17R-HDHA is a chemical compound available from Cayman Chemical. It serves as a research reagent. The core function of 17R-HDHA is to act as a precursor or intermediate in chemical reactions and studies. No further details on its intended use are provided.

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Lab products found in correlation

Multiquant 2, by AB Sciex (1 mentions) Arachidonic acid, by Cayman Chemical (1 mentions) Docosahexaenoic acid, by Cayman Chemical (1 mentions) 15 hete, by Cayman Chemical (1 mentions) Leukotriene b4, by Cayman Chemical (1 mentions) C8 precolumn, by Phenomenex (1 mentions) Prostaglandin e2, by Cayman Chemical (1 mentions) Protectin dx, by Cayman Chemical (1 mentions)

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HDHAs (2 citations)

2 protocols using 17r hdha

Profiling Lipid Mediators by LC-MS/MS

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LC-MS/MS-based lipid mediator and oxylipin profiling were carried out as described elsewhere [4 (link)]. A QTrap 6500 mass spectrometer (Sciex) was used, coupled to a Shimadzu Nexera LC30-system including auto-sampler and column oven (Shimadzu). The column was a Kinetex C18 50 × 2.1 mm, 1.7 μm, protected with a C8 pre-column (Phenomenex). LC-MS/MS peaks were integrated with manual supervision, and the areas were corrected to corresponding IS using MultiQuant™ 2.1 (Sciex). For quantitation, the multiple reaction monitoring (MRM) transitions and collision energies (CE) were used together with calibration lines for quantification. Calibration lines were constructed using 15-HETE, 17R-HDHA, leukotriene B₄, prostaglandin E₂, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic (Cayman Chemicals)

Brouwers H., von Hegedus J.H., van der Linden E., Mahdad R., Kloppenburg M., Toes R., Giera M, & Ioan-Facsinay A. (2022). Hyaluronidase treatment of synovial fluid is required for accurate detection of inflammatory cells and soluble mediators. Arthritis Research & Therapy, 24, 18.

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SPM Precursors and SPMs Administration

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SPM precursors and SPMs were injected as previously described at a concentration that leads to elevated levels of 14-HDHA, 17-HDHA, and PDX.^{43 (link)} Briefly, 14(S)-HDHA (0.1 mg/ml), 17R-HDHA (0.1 mg/ml), and Protectin DX (0.1 mg/ml) were purchased from Cayman Chemicals (Ann Arbor, MI). SPMs were prepared in ethanol in the dark and kept on ice at all times. The final cocktail concentration was 900 ng per mouse (300 ng of each SPM precursor and SPM) in PBS or vehicle control (ethanol in PBS) and was administered i.p. to lean and obese mice for 4 consecutive days followed by euthanasia on day 5. Control mice were administered a vehicle control.

Brito B., Martínez M., Fernández D., Rey L., Cabrera E., Palacios J.M., Imperial J, & Ruiz-Argüeso T. (1997). Hydrogenase genes from Rhizobium leguminosarum bv. viciae are controlled by the nitrogen fixation regulatory protein NifA. Proceedings of the National Academy of Sciences of the United States of America, 94(12), 6019-6024.

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