The following antibodies were used: cleaved Notch1 (Val1744, rabbit; Cell Signaling) 1:100 immunohistochemistry (IHC), 1:150 immunofluorescence (IF); Notch1 (D1E11, Cell Signaling) 1:1,000 Western blot (WB); cleaved Notch2 (cleaved-Ala1734, rabbit; Sigma) 1:150 IHC; cleaved Notch2 (cat. no. 07-1234, rabbit; Millipore) 1:200 IF, 1:1,000 WB; Snail1 (NBP1-19529, rabbit; Novus Biologicals) 1:200 IHC, 1:200 IF, 1:1,000 WB; Nephrin (guinea pig; Fitzgerald Laboratories) 1:250 IF; podocin (H-130, rabbit; Santa Cruz Biotechnology) 1:1,000 WB; Wilm's tumor suppressor gene 1 (WT-1) (C-19, mouse; Santa Cruz Biotechnology) 1:150 IF; GAPDH (clone GAPDH-71.1, mouse; Sigma) 1:5,000 WB; β-actin (clone AC-15, mouse; Sigma) 1:10,000 WB; and γ-tubulin (ab11316, mouse; Abcam) 1:1,000 WB. The following secondary antibodies were used: rhodamine-conjugated goat α guinea pig (Fitzgerald Laboratories) and fluorescein isothiocyanate–conjugated immunoabsorbed donkey α rabbit (The Jackson Laboratory). Nuclei were visualized with either Hoechst 33342 or NucBlue Fixed Cell stain (Life Technologies).
γ tubulin ab11316
Manufactured by Abcam
γ-tubulin (ab11316) is a laboratory reagent that is a member of the tubulin family of proteins. Tubulins are the main structural components of microtubules, which are critical for cellular processes such as cell division and intracellular transport. γ-tubulin is involved in the nucleation and organization of microtubules.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
β actin clone ac 15, by Merck Group (1 mentions)
Nucblue fixed cell stain, by Thermo Fisher Scientific (1 mentions)
Notch1 d1e11, by Cell Signaling Technology (1 mentions)
Gapdh clone gapdh 71, by Merck Group (1 mentions)
Flag m2 f1804, by Merck Group (1 mentions)
2 protocols using γ tubulin ab11316
1
Antibody Validation for Notch Signaling
2
Western Blot Protocol for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blots were performed under standard conditions using whole cell extracts and probed with the following antibodies: FLAG (M2 F1804; Sigma, St. Louis MO); Chd1 (39729; Active Motif, Carlsbad CA); γ-Tubulin (ab11316; Abcam, Cambridge MA).
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