Ab55268

Mouse monoclonal antibody for 3β‐HSD type 1 (Ab55268) was purchased from Abcam (Cambridge, UK). This antibody was raised against the recombinant full‐length protein, corresponding to amino acids 1–374 of human HSD3B1. Mouse IgG1‐kappa monoclonal antibody (ab18447) for isotype control was purchased from Abcam. Monoclonal antibodies for ER (SP1), PgR (1E2) and HER‐2 (4B5) were purchased from Ventana (Tucson, AZ, USA).

The paraffin sections and cultured LCs were incubated with rabbit polyclonal anti-HSD3B1 antibodies (ab55268, Abcam, Massachusetts, USA). After washing, the slides were incubated with biotinylated goat anti-rabbit IgG (ab64256, Abcam, Massachusetts, USA) for 1 h. After rinsing in phosphate-buffered saline (PBS), DAB (ab64261, Abcam, Massachusetts, USA) was used for the visualization of peroxidase activity. The slides were examined microscopically by a light microscope (DP73, Olympus, Tokyo, Japan).

Mouse monoclonal antibodies against 3βHSD1 (1:2,000, ab55268) and rabbit polyclonal antibodies against phospho-BMX (1:2,000, ab59409) were purchased from Abcam. Mouse monoclonal antibodies against phospho-tyrosine (pTyr) (1:2,000, 05-1050) were purchased from Sigma-Aldrich. custom-made rabbit monoclonal antibodies against phospho-3βHSD1 Y344 (1:2,000) were ordered from Affinity Biosciences. Mouse monoclonal antibodies against GST (1:5,000, AE001) were purchased from Abclonal. Mouse monoclonal antibodies against Flag (1:5,000, F3165) and anti-Flag M2 affinity gel (A2220) were purchased from Sigma-Aldrich. Rabbit monoclonal antibodies against HA (1:3,000, 3724S), β-actin (1:3,000, 3700S), and rabbit polyclonal antibodies against BMX (1:3,000, 24773) and GAPDH (1:5,000, 14C10) were obtained from Cell Signaling Technology.