UV-vis absorption spectra were recorded on a Shimadzu UV-vis1800 (Japan). The wavelength range, 200–600 nm, was used for data collection. All fluorescence spectra were recorded on Perkin Elmer LS55 Fluorescence (U.S.A.) spectrophotometer. The excitation wavelength was set at 280 nm and emission data was collected between 300 and 500 nm. Excitation and emission slit widths were kept at 10.0 nm. Molecular docking was carried out using Autodock Vina (Maltas and Ozmen, 2015 (link)).
Two milliliters of individual reactions were set up for UV-Vis data collection. Fluorescence data was collected by successively titrating dye stock solution manually, and mixing the reaction by pipetting.
Two milliliters of individual reactions were set up for UV-Vis data collection. Fluorescence data was collected by successively titrating dye stock solution manually, and mixing the reaction by pipetting.