The wheat seeds were kindly provided by a local farmer in Lingbi County, Suzhou City, Anhui Province, China. Hoagland Nutrition reagent was purchased from Qingdao Hope Bio-Technology Co., LTD (Qingdao, China). Polyethylene glycol 6000 was bought from Wuxi Yatai Allied Chemicals Co., Ltd. Glucose was from Shanghai Zhanyun Chemical Co., Ltd (Shanghai, China). Vanillin was from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Ursolic acid, Folin–Ciocalteu's phenol reagent, gallic acid, rutin hydrate, (+)-catechin, L-ascorbic acid, ABTS, DPPH were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). (±)-6-Hydroxy 2,5,7,8-tetramethylchromane-2-Carboxylic acid (Trolox) was from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All the reagents were of analytical grade.
Folin ciocalteu s phenol reagent
Manufactured by Maclin Biochemical Technology
Sourced in China
Folin–Ciocalteu's phenol reagent is a laboratory chemical used for the determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes. The reagent reacts with phenolic compounds, producing a blue color that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Maclin Biochemical Technology (1 mentions)
Catechin, by Maclin Biochemical Technology (1 mentions)
Rutin hydrate, by Maclin Biochemical Technology (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Maclin Biochemical Technology (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Maclin Biochemical Technology (1 mentions)
L ascorbic acid, by Maclin Biochemical Technology (1 mentions)
Vanillin, by Sinopharm (1 mentions)
Fecl3 6h2o, by Maclin Biochemical Technology (1 mentions)
Lacticacid, by Shanghai Lingfeng Chemical Reagent (1 mentions)
Malonic acid, by Maclin Biochemical Technology (1 mentions)
Glucose, by Maclin Biochemical Technology (1 mentions)
Sucrose, by Maclin Biochemical Technology (1 mentions)
Xylitol, by Adamas (1 mentions)
Malic acid, by Maclin Biochemical Technology (1 mentions)
2 4 6 tri 2 pyridyl s triazine tptz, by Merck Group (1 mentions)
Gallic acid, by Energy Chemical (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt abts, by Merck Group (1 mentions)
D sorbitol, by Maclin Biochemical Technology (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
Na2co3, by Sinopharm (1 mentions)
4 protocols using folin ciocalteu s phenol reagent
1
Wheat Antioxidant Properties Evaluation
2
Antioxidant Evaluation of Liverwort Extract
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Formic acid (HPLC grade), acetonitrile (ACN; HPLC grade), and gelatin (gel strength ~250 g Bloom) were purchased from Rhawn (Shanghai, China), 3,4,5-Trihydroxybenzoic acid and Na2CO3 were obtained from Bide Pharmatech Co., Ltd. (Shanghai, China). Folin-Ciocalteu’s phenol reagent, 2,4,6-Tri(2-pyridyl)-S-triazine, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), K2S2O8, and FeCl3·6H2O were purchased from Macklin (Shanghai, China).
The liverwort M. polymorpha was collected from Yandang Mountain, Wenzhou, China. The sterile thallus of M.polymorpha was obtained in the laboratory of Shandong University.
The liverwort M. polymorpha was collected from Yandang Mountain, Wenzhou, China. The sterile thallus of M.polymorpha was obtained in the laboratory of Shandong University.
3
Green Chemistry Extraction of Catechins
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All the solvents or reagents used were of HPLC or analytical grade. Ethanol, FeCl3·6H2O, HCl, K2S2O8, and acetic acid were purchased from Titan Scientific Co., Ltd. (Shanghai, China). Gallic acid was purchased from Energy-Chemical Co., Ltd. (Shanghai, China). ChCl and xylitol were obtained from Adamas Co., Ltd. (Shanghai, China). Na2CO3 was purchased from Sinopharm Chemical Reagent (Shanghai, China). D-sorbitol, glucose, maleic acid, malonic acid, malic acid, sucrose, and Folin–Ciocalteu’s phenol reagent were all obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Citric acid, ethylene glycol (EG), and 1,2-propanediol (PD) were purchased from TCI Shanghai (Shanghai, China). Lactic acid and sodium acetate were purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). Glycerol was purchased from MP Biomedicals Co., Ltd. (Shanghai, China). 2, 4, 6-Tri(2-pyridyl)-s-triazine (TPTZ), [2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma Aldrich (St. Louis, MO, USA). Commercial standards of catechins [(−)-EC, (−)-EGC, (−)-ECG, and (−)-EGCG, purity ≥ 98%] were purchased from Chengdu RefMedic Biotech Co., Ltd. (Chengdu, China).
4
Polyphenol Analysis of Fruit and Vegetables
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DPPH, ABTS, 2,4,6-tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), Folin–Ciocalteu's phenol reagent and chloroauric acid (40–50% Au based) were purchased from Macklin (Shanghai, China). All polyphenol standards including gallic acid, protocatechuic acid, caffeic acid, vanillic acid, ferulic acid, p-coumaric acid, apigenin, luteolin, rutin, quercetin, epigallocatechin (EGC) and epigallocatechin gallate (EGCG) were purchased from the Spring & Autumn Biological Engineering (Nanjing, China) with purity over 98%. Standard stock solutions of the polyphenol standards were prepared with methanol. Analytical grade methanol, ethanol, hydrochloric acid and acetic acid were purchased from Beijing Chemical Factory (Beijing, China). Ferrous sulfate heptahydrate, potassium persulfate, anhydrous sodium dihydrogen phosphate, sodium carbonate, sodium acetate trihydrate and ferric chloride were all analytical grade purchased from the Sino Pharm Chemical Reagent (Shanghai, China). Water was purified using Milli-Q from Millipore (Bedford, MA, USA). Raspberries (Rubus idaeus L.), apples (Malus domestica), grapes (Vitis vinifera cv. Kyoho), tangerines (Citrus reticulata Blanco), red peppers (Capsicum annuum sp.) and tomatoes (Solanum lycopersicum) used in the study were purchased from Wumei Supermarket (Beijing, China).
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