P mir 711

The plasmid encoding the ApN gene (p-ApN) was constructed by inserting the mouse full-length ApN cDNA45 (link) into the pcDNA™3.1D/V5-His-TOPO^® vector (pcDNA™3.1 Directional TOPO^® Expression Kit from Invitrogen, Thermo Fisher Scientific, Gent, Belgium). An empty plasmid was used as control. The plasmid containing the precursor miRNA for Mus musculus miR-711 stem-loop with Enhanced Green Fluorescent Protein (EGFP) reporter gene (p-miR-711) as well as its negative control were purchased (GeneCopoeia, Rockville, USA). All plasmids were amplified in Escherichia coli top 10F’ (Invitrogen), purified with an EndoFree plasmid giga kit (Qiagen, Venlo, The Netherlands), and then were stocked at −80 °C.

The plasmid encoding the ApN gene (p-ApN) was constructed by inserting the mouse full-length ApN cDNA [33 (link)] into the pcDNA™3.1D/V5-His-TOPO® vector (pcDNA™3.1 Directional TOPO® Expression Kit from Invitrogen, Thermo Fisher Scientific, Gent, Belgium) [11 (link)]. An empty plasmid was used as the control. The plasmid containing the precursor miRNA for Mus musculus miR-711 stem-loop with enhanced green fluorescent protein (EGFP) reporter gene (p-miR-711) as well as its negative control were purchased (GeneCopoeia, Rockville, MD, USA). All plasmids were amplified in Escherichia coli top 10 F′ (Invitrogen), purified with an EndoFree Plasmid Giga Kit (Qiagen, Venlo, The Netherlands) and then stocked at −80 °C.