Pancreatin

Pumpkin seeds (Cucurbita pepo L.) were provided by Haichuansanxin Food Company (Beitun, China). Alcalase (≥200 U/mg) was purchased by Hefei Bomei Biotechnology Co., Ltd. (Hefei, China). Papain (>200 units/mg) and flavourzyme (30,000 units/g) were purchased from Solarbio Life Science (Beijing, China). Bromelain (3,000–7,000 U/mg), pepsin (1,200 U/g), pancreatin (≥4,000U/g), trypsin (≥50,000U/g), and bile salt and o-Phthalaldehyde (OPA) and dithiothreitol (DTT) were purchased from Sangon Biotech (Shanghai, China). In addition, 8-Anilino-1-naphthalenesulfonic acid (ANS) [≥97% (HPLC)] was purchased from Sigma-Aldrich. Sodium dodecyl sulfate (SDS) and L-Serine (≥BR) were purchased from Sinopharm Chemical Reagent Co., Ltd. Zinc sulfate heptahydrate, sodium hydroxide, nitric acid, n-hexane, ethyl alcohol, and other reagents were of analytical grade and purchased from Chemical Reagent Co., Ltd. (Shanghai, China). Dialysis bags (MD34-500) were purchased from Wuxi Hengkang Medical Technology Co., Ltd.

Exenatide (EXE) was purchased from Chinese Peptide Company Co., Ltd. (Hangzhou, Zhejiang, China). Zein, casein, cholic acid (CA, 98%) and glycocholic acid hydrate (GCA, ≥ 97%) were from Sigma-Aldrich (Shanghai, China). Hypromellose phthalate (HP, HP-55S) was from Huzhou Mizuda Hope Bioscience Co., Ltd. (Huzhou, Zhejiang, China), and was purified by dialysis and lyophilization. Phosphatidylcholine (PC, egg yolk lecithin PL-100 M, 80% phosphatidylcholine) was from A.V.T. Pharmaceutical Co., Ltd. (Shanghai, China). Deoxycholic acid (DCA, > 99%), rhodamine B isothiocyanate (RITC, conjugation grade), pepsin (from porcine stomach, 3000–3500 NFU/mg), pancreatin (pancreatin-8.0, from porcine pancreas, protease 214 USP.U/mg, amylase 214 USP.U/mg and lipase 24.2 USP.U/mg), colchicine (reagent grade) and chlorpromazine (> 98%) were from Sangon Biotech Co., Ltd. (Shanghai, China). Sulfo-cyanine 5 NHS ester (Cy5, analytical grade) was from Lumiprobe Corporation (Maryland, USA). Fluorescein isothiocyanate (FITC) was from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). All culture reagents and materials were from Thermo Fisher Scientific Inc. (Shanghai, China). All other reagents were from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).