Log In Sign up
The largest database of trusted experimental protocols

Calam

Manufactured by Tecan
Visit Supplier
Sourced in United States

The CalAM is a compact automated sample identification and handling system designed for use in clinical and research laboratories. It is capable of processing sample tubes and microplates with a high degree of precision and efficiency. The CalAM's core function is to automate the sample identification and handling process, reducing the risk of manual errors and improving overall workflow productivity.

Automatically generated - may contain errors

Lab products found in correlation

Calam, by Thermo Fisher Scientific (2 mentions) Calcein am, by Thermo Fisher Scientific (2 mentions) Infinite f200, by Tecan (2 mentions)

2 protocols using calam

1

Islet Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
PPIs (100 IEQ) were washed with phosphate buffered saline and incubated for 30 min in Calcein AM (CalAM, Invitrogen, Carlsbad, Califonria, USA, cat#C1430) and propidium iodide (PI, Invitrogen, cat#P3566, Carlsbad, Califonria, USA) for staining of living and dead cells, respectively23 (link). After washing, the viability of CalAM/PI-stained islets was evaluated using a microplate reader (Tecan Infinite F200; Tecan, Chapel Hill, North Carolina, USA) and calculated using the following equation: CalAM-positive cells/(CalAM-positive cells + PI-positive cells) × 100.
Lau H., Khosrawipour T., Alexander M., Li S., Mikolajczyk A., Nicpon J., Schubert J., Bania J., Lakey J.R, & Khosrawipour V. (2020). Islet Transplantation in the Lung via Endoscopic Aerosolization: Investigation of Feasibility, Islet Cluster Cell Vitality, and Structural Integrity. Cell Transplantation, 29, 0963689720949244.
+ Open protocol
+ Expand
2

Islet Viability Assessment via Calcein AM and PI

Check if the same lab product or an alternative is used in the 5 most similar protocols
On day 7 of tissue culture, 100 IEQ were stained for 30 minutes at room temperature with Calcein AM (CalAM, Invitrogen, cat#C1430) to detect live islets and propidium iodide (PI, Invitrogen, cat#P3566) to detect dead or dying islets [17 (link)]. Stained islets were evaluated on a microplate reader (Tecan Infinite F200; Tecan) and the viability was calculated by the following equation:
CalAM−positivecells/(CalAM−positivecells+PI−positivecells)×100.
Lau H., Corrales N., Rodriguez S., Luong C., Mohammadi M., Khosrawipour V., Li S., Alexander M., de Vos P, & Lakey J.R. (2020). Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets. PLoS ONE, 15(12), e0243506.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!