The intestines were accessed via a laparotomy, excised, and pinned on to transparent silicone rubber (Sylgard 184; Dow Corning, Midland, MI, USA) then immersed in PSS within a dissection chamber maintained at 4°C. Individual MAs [inner diameter (ID), ~150 μm] supplying the small intestine were dissected and transferred to a tissue chamber (RC-27N, volume ~1 mL; Warner Instruments, Hamden, CT, USA) secured on an aluminum platform (length: 24 cm; width: 14.5 cm; thickness: 0.4 cm). Cannulation pipettes were pulled (P-97; Sutter Instruments, Novato, CA, USA) from glass capillaries (Cat. #64–1781, Warner; 0.94 mm ID, 1.2 mm outer diameter) and heat-polished (outer diameter, 100 μm). Each pipette was secured in a custom holder (Warner) held in a three-axis micromanipulator (DT3-100; Siskiyou Corp., Grants Pass, OR, USA) secured to the aluminum platform at each end of the tissue chamber. Pipettes were positioned in the chamber; individual MAs were cannulated and secured at each end with a strand of silk suture.22 (link),23 (link)
Rc 27n
Manufactured by Warner Instruments
Sourced in United States
The RC-27N is a laboratory equipment designed for perfusion and superfusion applications. It features a robust stainless steel construction and accommodates a range of chamber sizes to suit various experimental requirements. The device provides precise control over the flow rate of solutions, enabling researchers to maintain consistent and stable conditions during their experiments.
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Lab products found in correlation
2 protocols using rc 27n
1
Microvascular Cannulation of Intestinal Arteries
2
Isolation and Characterization of Cardiomyocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Live CM were imaged post-isolation using a Leitz Laborlux 11 microscope (Leica Microsystems, Vienna, Austria) with a 2.5× air objective and an eyepiece camera (MikrOkular, Bresser, Rhede, Germany). To assess the yield of rod-shaped CM, we counted rod-shaped and dead (rounded) CM in at least three fields of view per isolation (60–500 CM manually counted per field of view). To assess contractile function, we paced CM in a perfusion chamber equipped with field stimulation electrodes (RC-27N, Warner Instruments, Hamden, MA, USA), driven by a MyoPacer field stimulator (applied voltage was twice the threshold and maximally 12 V, pulse duration 5 ms; pacing frequency was 0.5 Hz for ventricles and 1 Hz for atria; IonOptix, Westwood, MA, USA). Fractions of rod-shaped and contracting CM (i.e., responding to field stimulation without arrhythmic events, e.g., pauses, extra contractions) are expressed as percent of all CM in a field of view.
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