To achieve the in vitro manipulation of miR-136, human trophoblast cell lines HRT-8/SVneo and BeWo were transfected with miR-136 mimics, miR-136 inhibitors, or negative controls (mimic NC and inhibitor NC) (GenePharma, Shanghai, China) by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). In addition, an overexpression vector of E2F1 pcDNA3.1-E2F1 was synthesized by GenePharma (Shanghai, China) and was transfected into trophoblast cells using Lipofectamine 2000 (Invitrogen). The cell transfection experiment was performed according to the manufacturer's instruction. The subsequent cell analyses were carried out at 48 h posttransfection.
Mimic nc and inhibitor nc
Manufactured by GenePharma
Sourced in China
Mimic NC and inhibitor NC are cell transfection reagents designed for the effective delivery of small interfering RNA (siRNA) or microRNA (miRNA) molecules into mammalian cells. These products facilitate the study of gene function and regulation by enabling the modulation of target gene expression. The core function of these reagents is to efficiently transport the desired RNA molecules into cells, allowing for the investigation of their biological effects.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Mir 627 3p inhibitor, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Si nc, by GenePharma (1 mentions)
Si neat1, by GenePharma (1 mentions)
Si nr4a2, by GenePharma (1 mentions)
Nc sirna, by Thermo Fisher Scientific (1 mentions)
Mir 128 3p inhibitor, by GenePharma (1 mentions)
Sirt1 sirna, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
5 protocols using mimic nc and inhibitor nc
1
In Vitro Manipulation of miR-136 in Trophoblast Cells
2
Transfection Efficiency Optimization
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The transfection reagents IFNG-AS1 siRNA (si-IFNG-AS1) and siRNA negative control (siRNA-NC), miR-627-3p mimic, miR-627-3p inhibitor, and corresponding NCs (mimic NC and inhibitor NC) were all obtained from GenePharma (Shanghai, China). After being incubated in 6-well plates and grown to a cell density of 50%, the cells were transfected with the transfection reagents for 24 h with the help of Lipofectamine 2000 reagent [18 (link)]. Transfection efficiency was measured using reverse transcription-quantitative PCR (RT-qPCR) 48 h post-transfection. The control group was the untreated cells.
3
DANCR Silencing via siRNA Transfection
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Transfection was initiated upon achieving a cell fusion rate reached of 70%. Small interfering (si) RNA targeting DANCR (si-DANCR) and its negative control (si-NC) or, as well as the miR-214-5p mimic, miR-214-5p inhibitor, and their negative control (mimic NC and inhibitor NC, obtained from GenePharma, China), were combined with the transfection reagent Lipofectamine 3000 (cat: 1,662,152, Invitrogen, USA) in DMEM. The mixture was then allowed to incubate for 20 min at room temperature. Subsequently, the prepared mixture was introduced into the cells, and the incubation continued for 6 h before replacing the DMEM.
4
Regulation of NEAT1 and NR4A2 by miR-511 in HNECs
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Small interfering RNAs (siRNAs) targeting NEAT1 (si-NEAT1) and NR4A2 (si-NR4A2) with their negative control (si-NC), and miR-511 mimics and miR-511 inhibitor with their negative control (NC mimic and NC inhibitor) were obtained from GenePharma (Shanghai, China). HNECs (1 x 105) were transfected with si-NEAT1, si-NR4A2, si-NC, miR-511, NC mimics, miR-511 inhibitor or NC inhibitor using Lipofectamine 2000 (Invitrogen, USA). HNECs were collected 48 h after transfection according to previous study [18 ].
5
Overexpression of KCNQ1OT1 in chondrocytes
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The gene-overexpression vector (Ad-KCNQ1OT1) and the control vector (Vector) were purchased from GenScript Biotech Corp. (Nanjing, China). The small interfering RNAs against SIRT1 (SIRT1 siRNA) and negative control siRNAs (NC siRNA) were designed, synthesized and validated by Thermo Fisher Scientific (Waltham, MA, USA). MiR-128a-3p mimic, miR-128-3p inhibitor and the corresponding negative control (NC mimic and NC inhibitor) were designed and synthesized by GenePharma Corporation (Shanghai, China). All these plasmids and oligonucleotides were transfected into ATDC5 chondrocyte cells by using lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, USA) following the guidelines of the manufacturer. At 48 h after transfection, cells were harvested for further study.
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