Non-mobilized apheresis was obtained from healthy donors at the Bone Marrow Transplant and Cell Therapy Unit (BMTCT) of Hospital Universitario La Paz (HULP) by using CliniMACS Plus device (Miltenyi Biotec). All donors gave their written informed consent in accordance with the Declaration of Helsinki protocol, and the study was performed according to the guidelines of the local ethics committee. All donors comply with the requirements regarding quality and safety for donation, obtaining, storage, distribution, and preservation of human cells and tissues under the Spanish specific regulation. CD45RA+ cells were depleted by immunomagnetic separation using CliniMACS CD45RA Reagent (701-46) and CliniMACS Plus system, both from Miltenyi Biotec, following manufacturer instructions. CD45RA− cells were either processed immediately or stored at 2–8°C for subsequent processing no later than 24 h after depletion. The viability and purity of CD45RA− fraction were analyzed by flow cytometry (FCM) before activation, transduction, and expansion.
Clinimacs plus device
Manufactured by Miltenyi Biotec
The CliniMACS Plus device is a laboratory instrument designed for the separation of target cells from a heterogeneous cell population. It utilizes magnetic cell separation technology to isolate specific cell types, which can be used for various applications in research and clinical settings.
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Lab products found in correlation
2 protocols using clinimacs plus device
1
CD45RA-Depleted Cell Isolation and Processing
2
PBMC Apheresis and CD4/CD8 Selection
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PBMC apheresis products were collected according to standard procedures. Apheresis cells were counted, and flow cytometry was performed to evaluate the frequencies of cells expressing CD3, CD4, CD8, and CD19 prior to CD4 plus CD8 T cell selection. For CD4 plus CD8 selection, apheresis products were labeled with anti-CD4 and anti-CD8 microbeads, and column purification was performed with a CliniMACS Plus device (Miltenyi Biotec).
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