For N-glycan release and analysis by liquid chromatography, the following materials and reagents were used. AcroPrep Advance 96-filter plates and 10 kDa MWCO microcentrifuge filtration tubes were acquired from Pall Life sciences (USA). Polypropylene 2 mL deep 96-well blocks were purchased from Fisher Scientific (Ireland) and 0.45 μm Millex-LH filters from Millipore (Ireland). Plate seals were obtained from Cruinn (Ireland) and silicone sealing mats from Phenomenex (Germany). Protogel was purchased from National Diagnostics (UK) and ammonium hydroxide solution was acquired from Fluka (Ireland). Formic acid and ammonium persulfate (APS) were obtained from VWR chemicals (Ireland). Ultrapure water was filtered through an arium® ProUV system (Sartorius, Germany). HPLC grade methanol, ethanol, acetonitrile, xylene and water were acquired from Fisher Scientific (Ireland). PNGase F was purchased from New England Biolabs. All other reagents were purchased from Sigma (including N,N,N′,N′-tetramethylethane-1,2-diamine (TEMED), iodoacetamide (IAA), dithiothreitol (DTT) and 2-aminobenzamide (2-AB)) unless otherwise specified.
Arium prouv system
Manufactured by Sartorius
Sourced in Germany
The Arium® ProUV system is a water purification system designed to produce high-quality, ultrapure water. It utilizes a combination of filtration and UV irradiation to remove impurities and ensure the water meets the required purity standards for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Silicone sealing mats, by Phenomenex (2 mentions)
Formic acid, by Avantor (2 mentions)
Protogel, by National Diagnostics (2 mentions)
Pngase f, by New England Biolabs (2 mentions)
Xylene, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Ammonium persulfate aps, by Avantor (1 mentions)
Millex lh filter, by Merck Group (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Ammonium persulfate, by Avantor (1 mentions)
Tcs 2002, by Bio-Techne (1 mentions)
Indirubin, by Merck Group (1 mentions)
Roscovitine, by Selleck Chemicals (1 mentions)
4 protocols using arium prouv system
1
N-Glycan Release and Liquid Chromatography
2
N-Glycan Release and Analysis by LC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For N-glycan release and analysis by liquid chromatography, the following materials and reagents were used. AcroPrep Advance 96-lter plates and 10 kDa MWCO microcentrifuge ltration tubes were acquired from Pall Life sciences (US). Polypropylene 2 mL deep 96-well blocks were purchased from Fisher Scienti c (Ireland) and 0.45 µm Millex-LH lters from Millipore (Ireland). Plate seals were obtained from Cruinn (Ireland) and silicone sealing mats from Phenomenex (Germany). Protogel was purchased from National Diagnostics (UK) and ammonium hydroxide solution was acquired from Fluka (Ireland). Formic acid and ammonium persulfate (APS) were obtained from VWR chemicals (Ireland). Ultrapure water was ltered through an arium® ProUV system (Sartorius, Germany). HPLC grade methanol, ethanol, acetonitrile, xylene and water were acquired from Fisher Scienti c (Ireland). PNGase F was purchased from New England Biolabs. All other reagents were purchased from Sigma (including N,N,N',N'tetramethylethane-1,2-diamine (TEMED), iodoacetamide (IAA), dithiothreitol (DTT) and 2aminobenzamide (2-AB)) unless otherwise speci ed.
3
Intrahippocampal Pilocarpine Administration Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The potent and selective GSK-3β inhibitor TCS2002 was purchased from Tocris Bioscience (Bristol, United Kingdom) and the chemoconvulsant pilocarpine.HCl from Sigma (St Louis, MO, USA). All other chemicals were analytical reagent grade and were obtained from Merck (Darmstadt, Germany). Aqueous solutions were prepared freshly, with water purified by an Arium pro UV system (Sartorius Stedim Biotech GmbH, Goettingen, Germany). For the in vivo experiments with intrahippocampal administration of pilocarpine, stock solutions were prepared in ultrapure water, diluted with modified Ringer's solution (147 mM NaCl; 4 mM KCl; 2.3 mM CaCl2), previously filtered through a membrane filter (0.2 µm diameter) and directly delivered into the hippocampus through perfusion of a microdialysis probe. Prior to the systemic administration, TCS2002 was dissolved in propylene glycol:saline (50:50).
4
Evaluation of Neuroprotective Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Indirubin was obtained from Sigma-Aldrich Chemicals (Bornem, Belgium), BIO-acetoxime was purchased from Tocris Bioscience (Bristol, United Kingdom) and chosen because of its high inhibitory activity towards GSK-3, roscovitine from Selleckchem (Houston, USA), pilocarpine.HCl and PTZ from Sigma (St Louis, MO, USA). All other chemicals were analytical reagent grade and were obtained from Merck (Darmstadt, Germany). Aqueous solutions were prepared in fresh water purified by an arium pro UV system (Sartorius Stedim Biotech GmbH, Goettingen, Germany). Prior to their systemic administration in rodents, Indirubin and BIOacetoxime were dissolved in propyleenglycol:saline (50:50). Since the metabolic and chemical stability of the compounds is unknown, solutions were freshly prepared and syringes (protected from light) were filled ex tempore to minimize the influence of possible degradation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!