The allograft was fixed in 10% phosphate-buffered formalin, embedded in paraffin, and cut into 5-μm sections for hematoxylin‐eosin (HE) staining. The sections were then incubated with primary antibodies including anti‐CD4 Ab (Boster), anti‐CD8 Ab (Boster), anti-CD3 (Abcam Inc, Cambridge, MA, USA), anti-ki67 (Affinity, Changzhou, China), anti-actinin (Boster), anti-cleaved-caspase-3 (Affinity), anti-cleaved-PARP (Affinity), anti-IFN-γ (Affinity), and goat anti-rabbit IgG secondary antibodies (Boster). Images were captured with a Leica Aperio Versa 200 (Germany) whole slide imaging system. Two cardiologists blinded to the experimental conditions graded acute rejection according to the International Society of Heart and Lung Transplantation criteria (23 (link)).
Anti cd4 ab
Manufactured by Boster Bio
Sourced in United States
Anti-CD4 Ab is a monoclonal antibody that binds to the CD4 receptor on the surface of T helper cells. It is a laboratory tool used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd8 ab, by Boster Bio (2 mentions)
Anti cleaved caspase 3, by Affinity Biosciences (1 mentions)
Goat anti rabbit igg secondary antibody, by Boster Bio (1 mentions)
Anti cleaved parp, by Affinity Biosciences (1 mentions)
Anti ki67, by Affinity Biosciences (1 mentions)
Anti ifn γ, by Affinity Biosciences (1 mentions)
Anti cd3, by Abcam (1 mentions)
Aperio versa 200, by Leica (1 mentions)
Goat anti rabbit igg, by Boster Bio (1 mentions)
Dab detection kit, by Maixin Group (1 mentions)
Pannoramic p250, by 3DHISTECH (1 mentions)
2 protocols using anti cd4 ab
1
Histological Assessment of Cardiac Allograft
2
Histopathological Analysis of Xenograft and Allograft
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The xenograft and allograft were fixed in 10% phosphate-buffered formalin,
embedded in paraffin, and cut into 5 µm sections for hematoxylin and eosin
(H&E) staining. Immunohistochemical staining was performed as described previously17 (link)
, the sections were then stained with primary antibody anti-CD4 Ab
(Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM
(Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan,
China). Samples were visualized with a DAB detection kit (Maixin-Bio, Fuzhou,
China). We used a pathological section scanner (Pannoramic P250, 3DHISTECH,
Budapest, Hungary) to analyze the immunohistochemical staining density. Two
cardiologists blinded to the experimental conditions graded acute rejection
according to the International Society of Heart and Lung Transplantation (ISHLT) criteria21 (link)
. Briefly, 0 R = no rejection; 1 R (mild rejection) = evidence of
perivascular infiltrate, interstitial infiltrate, or both with up to 1 focus of
myocyte damage; 2 R (moderate rejection) = two or more infiltrate foci with
related myocyte damage; 3 R (severe rejection) = the infiltrate was diffuse and
had multifocal myocyte damage ± edema, ± hemorrhage, ± vasculitis.
embedded in paraffin, and cut into 5 µm sections for hematoxylin and eosin
(H&E) staining. Immunohistochemical staining was performed as described previously17 (link)
, the sections were then stained with primary antibody anti-CD4 Ab
(Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM
(Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan,
China). Samples were visualized with a DAB detection kit (Maixin-Bio, Fuzhou,
China). We used a pathological section scanner (Pannoramic P250, 3DHISTECH,
Budapest, Hungary) to analyze the immunohistochemical staining density. Two
cardiologists blinded to the experimental conditions graded acute rejection
according to the International Society of Heart and Lung Transplantation (ISHLT) criteria21 (link)
. Briefly, 0 R = no rejection; 1 R (mild rejection) = evidence of
perivascular infiltrate, interstitial infiltrate, or both with up to 1 focus of
myocyte damage; 2 R (moderate rejection) = two or more infiltrate foci with
related myocyte damage; 3 R (severe rejection) = the infiltrate was diffuse and
had multifocal myocyte damage ± edema, ± hemorrhage, ± vasculitis.
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