70 μm strainer

Manufactured by SPL Life Sciences

Sourced in United States

The 70 μm strainers are designed for filtering and separating samples. They feature a stainless steel mesh with a pore size of 70 micrometers, which is suitable for a variety of filtration applications.

Automatically generated - may contain errors

Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) First strand cdna synthesis kit, by Takara Bio (1 mentions) Hrp conjugated anti rabbit igg antibody, by Cell Signaling Technology (1 mentions) Il 17 elisa kit, by R&D Systems (1 mentions) Sybr premix ex taq, by Takara Bio (1 mentions) Ethanol, by Merck Group (1 mentions) Tumor dissociation kit, by Miltenyi Biotec (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Crl 2097, by American Type Culture Collection (1 mentions) Percoll, by GE Healthcare (1 mentions) Ack lysis buffer, by Thermo Fisher Scientific (1 mentions) Collagenase 4, by Worthington (1 mentions)

Close spelling variants of this product

70-μm cell strainer

4 protocols using 70 μm strainer

Immunomodulatory Effects of Topical Imiquimod

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Ethanol (Merck, Germany), acetone (Duksan, Korea), hair removal cream (Veet; Reckitt Benckiser, France), IMQ (Dong-a Otsuka, Korea), Vaseline (Unilever Korea, Korea), 70 μm strainers (SPL life science, Korea), IL-17 ELISA kits (R&D Systems MN, USA), and PerCP-Cy5.5-Rat IgG1,κ (RTK2071, Biolegend, USA) were used. First Strand cDNA Synthesis Kits and SYBR Premix EX Taq were purchased from Takara Bio (Japan).
CD16/CD32, fluorescein isothiocyanate (FITC)-anti-mouse CD4 (Mouse CTL clone V4) and FITC-Rat IgG2b,κ (TNP-Keyhole Limpet Hemocyanin) were purchased from BD Biosciences (USA). FITC-anti-mouse γδ TCR (UC7-13D5) and phycoerythrin (PE)-anti-mouse IL-17A, FITC-Armenian Hamster IgG (eBio299Arm), PE-anti-mouse IL-17A (eBio17B7), PerCP-Cy5.5-anti-mouse IFNγ (XMG1.2), PE-anti-Rat IgG2a/κ (eBR2a) and TRIzol reagent were purchased from Thermo Fisher Scientific (USA). Rabbit anti-STAT1(D1K9Y), rabbit anti-phospho-STAT1 (Tyr701), rabbit anti-STAT3 (79D7), rabbit anti-phospho-STAT3 (D3A6), rabbit anti-STAT5 (D2O6Y), rabbit anti-phospho-STAT5 (C11C5) and HRP-conjugated anti-rabbit IgG antibodies were purchased from Cell Signaling (USA). Mouse anti-β-Actin (C4) antibodies and HRP-conjugated m-IgGκ BP were purchased from Santa Cruz Biotechnology (USA).

Choi M., Yi J.K., Kim S.Y., Ryu J.H., Lee J., Kwon W., Jang S., Kim D., Kim M., Kim H., Kim S.H., Choi S.K, & Ryoo Z.Y. (2019). Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis. BMC Complementary and Alternative Medicine, 19, 347.

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Isolation of Immune Cells from Ear Tissues

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The spleen and cLN were prepared using 70 μm strainers (SPL Life Sciences, Gyeonggi-do, Korea). Ear tissues were cut into small pieces and digested at 37 °C for 40 min in RPMI (Roswell Park Memorial Institute)-1640 medium containing 100 μl of enzyme D, 50 μl of enzyme R, and 12.5 μl of enzyme A from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). After digestion, 2 ml of RPMI-1640 medium was added, and dissociation commenced by the gentleMACS program_B_01. Following dissociation, the tissues were passed through 70 μm strainers. After centrifugation, the resulting cell pellet was washed with sterile phosphate buffered saline (PBS), centrifuged, and resuspended in RPMI-1640 medium.

Min K.Y., Kim D.K., Jo M.G., Choi M.Y., Lee D., Park J.W., Park Y.J., Chung Y., Kim Y.M., Park Y.M., Kim H.S, & Choi W.S. (2024). IL-27-induced PD-L1highSca-1+ innate lymphoid cells suppress contact hypersensitivity in an IL-10-dependent manner. Experimental & Molecular Medicine, 56(3), 616-629.

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Isolation of Human Liver Fibroblasts

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Human skin fibroblasts (HSFs, CRL-2097) were
purchased from the American Type Culture Collection
(ATCC) for use as non-malignant control cells. In this
experimental study, human liver fibroblasts (HLFs) were
isolated from the liver specimen of a 69-year-old male
patient with cHCC-CC. All experiments were approved
by the Institutional Review Board (IRB) at Chungnam
National University Hospital (IRB file no. CNUH 2016-
03-018) and at Public Institutional Bioethics Committee
designated by the South Korea Ministry of Health and Welfare (IRB file no. P01-201703-31-010). The patient
provided informed consent. The human liver tissues
were washed with cold phosphate-buffered saline (PBS,
Thermo Fisher, USA), as previously described (30 (link)),
and minced in a solution that contained 300 units/ml
collagenase type IV (Thermo Fisher, USA). The minced
tissue was incubated at 37˚C for 30 minutes until they
were digested, then filtered through a 70 μm strainer (SPL
Life Science, Korea) and washed with a cold solution of
10% foetal bovine serum (FBS, Thermo Fisher, USA) in
PBS. The cells were resuspended in minimal essential
medium (MEM, Thermo Fisher, USA) containing 10%
FBS and 1% penicillin streptomycin (PS, Thermo Fisher,
USA).

Ahn H.S., Ryu J.S., Lee J., Mun S.J., Hong Y.H., Shin Y., Chung K.S, & Son M.J. (2022). Generation of An Induced Pluripotent Stem Cell Line from Human Liver Fibroblasts from A Patient with Combined Hepatocellular-Cholangiocarcinoma. Cell Journal (Yakhteh), 24(3), 133-139.

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Isolation of Liver and Lymphocyte Cells

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The liver was removed from mice after perfusion with DPBS via the hepatic portal vein. Perfused liver homogenates were incubated with collagenase IV (Worthington, NJ, USA) for 15–30 min at 37 °C, and were passed through a 100-μm strainer. Cells were centrifuged at 800× for 5 min and pellets were suspended in 33% Percoll (GE Healthcare Bio-Sciences, PA, USA). The suspension was centrifuged at 800 × g for 30 min and RBCs were removed with ACK lysis buffer (Gibco, MA, USA). Lymphocytes from spleens of WT and CD160^−/− mice were homogenized using a 70-μm strainer (SPL, Korea) to isolate single cells.

Kim T.J., Park G., Kim J., Lim S.A., Kim J., Im K., Shin M.H., Fu Y.X., Del Rio M.L., Rodriguez-Barbosa J.I., Yee C., Suh K.S., Kim S.J., Ha S.J, & Lee K.M. (2019). CD160 serves as a negative regulator of NKT cells in acute hepatic injury. Nature Communications, 10, 3258.

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