Exponentially growing cells in EZ-RDM+0.2% glucose at 30 °C were loaded into a B04a bacterial microfluidic chip in a CellASIC ONIX system (EMD Millipore, Hayward, CA, USA). Cells were grown in EZ-RDM+0.2% glucose for 10 min at 30 °C while being imaged in phase-contrast every 30 s. Then, the medium was switched to EZ-RDM+0.2% glucose with 100 μg ml−1 cefsulodin and imaging continued for another 50 min. Cell contours were automatically extracted from phase-contrast images using a custom MATLAB package. Cell length over time, L(t), was smoothed with a moving average window of 10 time points and instantaneous growth rate was calculated as 1/L dL/dt.
Cellasic onix system
Manufactured by Merck Group
Sourced in United States
The CellASIC ONIX system is a microfluidic cell culture platform that enables real-time monitoring and precise control of the cellular microenvironment. The system provides a stable and dynamic culture environment for live-cell imaging and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cellasic onix system
1
Measuring Cell Growth in Microfluidics
2
Live Cell Permeability Imaging
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