Uptake of L-[3H]amino acids (PerkinElmer Life Sciences) was measured in oocytes expressing wild-type and mutant ASCT1, and uninjected oocytes. Five oocytes were incubated in Cl−-containing buffer with 10 µM L-[3H]amino acids at room temperature. After 10 min, uptake was terminated by three rapid washes in ice-cold Cl−-containing buffer followed by lysis in 50 mM NaOH and 1% SDS. L-[3H]amino acids were measured by scintillation counting using a Trilux beta counter (PerkinElmer Life Sciences).
L 3h amino acids
Manufactured by PerkinElmer
L-[3H]amino acids are radiolabeled amino acids used in various research applications. They can be utilized as precursors for protein synthesis studies or as metabolic tracers. These amino acids are labeled with tritium (3H), a radioactive isotope of hydrogen, which allows for their detection and quantification in experimental systems.
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Lab products found in correlation
1 protocol using l 3h amino acids
1
Radiolabeled Amino Acid Uptake Assay
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