Allophycocyanin conjugated streptavidin

Manufactured by Thermo Fisher Scientific

Sourced in Japan

Allophycocyanin-conjugated streptavidin is a fluorescent protein complex used in various biological applications. Streptavidin, a protein derived from the bacterium Streptomyces, has a strong affinity for the small molecule biotin. Allophycocyanin, a fluorescent pigment found in certain algae, is covalently attached to the streptavidin, creating a fluorescent probe. This complex can be used to detect and quantify biotinylated molecules in experiments such as immunoassays, flow cytometry, and microscopy.

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Lab products found in correlation

Anti cd4 clone gk1, by Thermo Fisher Scientific (2 mentions) Anti pgk clone 22c5d8, by Thermo Fisher Scientific (2 mentions) Anti mouse human actin clone ab 5, by BD (2 mentions) Horseradish peroxidase conjugated anti mouse ig, by GE Healthcare (2 mentions) Anti tcrβ clone h57, by BD (2 mentions) Anti cd19 clone 1d4, by BD (2 mentions) Phycoerythrin conjugated streptavidin, by Southern Biotech (2 mentions) Horseradish peroxidase conjugated goat anti rabbit ig, by Southern Biotech (2 mentions) Anti ter119 clone ter 119, by BD (2 mentions) B cell isolation kit, by Miltenyi Biotec (2 mentions) Propidium iodide, by Merck Group (2 mentions) Optimum cutting temperature compound, by Sakura Finetek (1 mentions) Hoechst 33258, by Dojindo Laboratories (1 mentions) Pentobarbital, by Kyoritsu Seiyaku (1 mentions)

Spelling variants of this product

Streptavidin-allophycocyanin (11 citations) Allophycocyanin (APC)–conjugated streptavidin (2 citations)

3 protocols using allophycocyanin conjugated streptavidin

Antibodies and Biomolecules for Cellular Analysis

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Anti-PGK (clone 22C5D8, Invitrogen), anti-mouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma gondii actin^{39 (link)}, horseradish peroxidase-conjugated goat anti-rabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated anti-mouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19 (clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen), allophycocyanin-conjugated streptavidin (ebiosciences, cat. number 17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech, cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the experiments described here we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues, and with the following mutations: E105K/E108A^{40 (link)} and P94R/D160N/D165A/K190E/K196T^{24 (link)}: Sortase A was produced and purified as described elsewhere^{20 (link)}. Enhancer^{33 (link)} and VHH7^{34 (link)} were produced and purified as described elsewhere^{34 (link)}. Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG) was produced by the MIT biopolymer facility through standard solid phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere^{20 (link)}.

Swee L.K., Lourido S., Bell G.W., Ingram J.R, & Ploegh H.L. (2014). One-Step Enzymatic Modification of the Cell Surface Redirects Cellular Cytotoxicity and Parasite Tropism. ACS Chemical Biology, 10(2), 460-465.

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Engineered Sortase A for Bioconjugation

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Anti-PGK (clone 22C5D8, Invitrogen),
antimouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma
gondii actin,^{39 (link)} horseradish peroxidase-conjugated
goat antirabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated
antimouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone
H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19
(clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen),
allophycocyanin-conjugated streptavidin (ebiosciences, cat. number
17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech,
cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number
P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the
experiments described here, we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues,
and with the following mutations: E105 K/E108A^{40 (link)} and P94R/D160N/D165A/K190E/K196T:^{24 (link)} Sortase A was produced and purified as described elsewhere.^{20 (link)} Enhancer^{33 (link)} and VHH7^{34 (link)} were produced and purified as described elsewhere.^{34 (link)} Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG)
was produced by the MIT biopolymer facility through standard solid
phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere.^{20 (link)}

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Immune Cell Profiling in Mouse Ear Tissue

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Mice were anesthetized with pentobarbital (Kyoritsu Seiyaku Co., Tokyo, Japan) and were perfusion-fixed with 4% paraformaldehyde. Ears were then removed and cryoembedded in optimum cutting temperature compound (Sakura Finetek, Tokyo, Japan), and 10-μm sections were cut using a cryostat. Cryosections were refixed in 4% paraformaldehyde for 15 minutes and were incubated for 1 hour at room temperature in PBS containing skin milk. After blocking, cryosections were incubated for 1 hour with primary biotinylated antibodies specific for Gr-1, CD11c, and F4/80 (1:50 dilutions; eBioscience). Cryosections were then incubated for 30 minutes with allophycocyanin-conjugated streptavidin (1:100 dilution; eBioscience) and nuclei were stained using Hoechst 33258 (Dojindo Laboratories, Kumamoto, Japan).

Hattori H, & Ishihara M. (2015). Altered protein secretions during interactions between adipose tissue- or bone marrow-derived stromal cells and inflammatory cells. Stem Cell Research & Therapy, 6(1), 70.

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