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Mouse anti nestin

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The mouse anti-Nestin antibody is a primary antibody used to detect the expression of the Nestin protein, which is a type VI intermediate filament protein. Nestin is commonly used as a marker for neural stem and progenitor cells. This antibody can be utilized in various analytical techniques, such as immunocytochemistry and Western blotting, to identify and study Nestin-expressing cells.

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Lab products found in correlation

Rabbit anti vimentin, by Cell Signaling Technology (2 mentions) Rabbit anti oct4, by Abcam (2 mentions) Mouse anti tra 1 60, by Thermo Fisher Scientific (2 mentions) Rat anti k8, by Developmental Studies Hybridoma Bank (2 mentions) Rabbit anti lamin b1, by Abcam (2 mentions) Mouse anti gigaxonin, by Santa Cruz Biotechnology (2 mentions) Mouse anti keratin 8, by Thermo Fisher Scientific (2 mentions) Mouse anti tra 1 81, by Thermo Fisher Scientific (2 mentions) Mouse anti vimentin, by Thermo Fisher Scientific (2 mentions) Rabbit anti lamin a c, by Santa Cruz Biotechnology (2 mentions) Rabbit anti sox2, by Thermo Fisher Scientific (2 mentions) Rat anti mbp, by Abcam (2 mentions) Mouse anti cc1, by Abcam (2 mentions) Alexa 488 or alexa 594 conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (2 mentions) Mouse anti gfap, by Merck Group (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Goat anti lin28a, by R&D Systems (1 mentions) Mouse anti tra 1 81, by Merck Group (1 mentions) Rabbit anti pax6, by BioLegend (1 mentions)

Close spelling variants of this product

Nestin (22 citations) Anti-Nestin (8 citations) Anti-Nestin (mouse clone 10C2, (2 citations)

5 protocols using mouse anti nestin

1

Comprehensive Immunofluorescence and Western Blot Antibody Protocol

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The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell-Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-NF-M/H (clone RMdO-20; IF 1:100), mouse anti-Tra-1-60 (Thermo Fisher Scientific, 41-1000, IF 1:300), mouse anti-Tra-1-81 (Thermo Fisher Scientific, 41-1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48-1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat-, anti-mouse-, and rabbit-antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat-, anti-mouse-, and rabbit-antibodies (Sigma, WB 1:5000).
Battaglia R.A., Faridounnia M., Beltran A., Robinson J., Kinghorn K., Ezzell J.A., Bharucha-Goebel D., Bönnemann C.G., Hooper J.E., Opal P., Bouldin T.W., Armao D, & Snider N.T. (2023). Intermediate filament dysregulation in astrocytes in the human disease model of KLHL16 mutation in giant axonal neuropathy (GAN). Molecular Biology of the Cell, 34(12), ar121.
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2

Immunocytochemical Characterization of iPSC-Derived Motor Neurons

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Cultured human iPSCs or stem cell-derived motor neurons were fixed for 10 min in 4% PFA, then permeabilized and blocked for 1 hr in 1% goat serum and 0.1% Triton X-100 in PBS. Primary antibodies were applied overnight at 4°C in Shandon coverplates. Primary antibodies used for stem cell characterization include mouse anti-TRA-1–81 (Millipore, 1:200), rabbit anti-Oct4 (ThermoFisher, 1:1,000), goat anti-LIN-28A (R&D Biosystems, 1:50), mouse anti-Nanog (Santa Cruz, 1:200), rabbit anti-α fetoprotein (Dako, 1:400), mouse anti-α smooth muscle Actin (Sigma, 1:400), mouse anti-Nestin (ThermoFisher, 1:200), rabbit anti-PAX6 (BioLegend, 1:2000).
Primary antibodies used for motor neuron characterization include mouse anti-Islet-1/2 (DSHB, 1:500), mouse anti-HB9/Mnx1 (DSHB, 1:500), goat anti-SCIP (Santa Cruz, 1:5000), anti-HOXA5 (Dasen et al., 2005 (link)), anti-FOXP1 (Dasen et al., 2008 (link)), anti-HOXC6 (Liu et al., 2001 (link)), anti-HOXC9 (Jung et al., 2010 (link)), anti-LHX3 (Tsuchida et al., 1994 (link)), rabbit anti-OLIG2 (Proteintech, 1:500), mouse anti-p-Histone H3 (Santa Cruz, 1:400), Alexa 488 pre-conjugated rabbit anti-Cleaved Caspase-3 (Cell Signaling, 1:50). For detection, we used Alexa fluor [488, 555, 594, 647]-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (Invitrogen, 1:1,000).
LaForce G.R., Farr J.S., Liu J., Akesson C., Gumus E., Pinkard O., Miranda H.C., Johnson K., Sweet T.J., Ji P., Lin A., Coller J., Philippidou P., Wagner E.J, & Schaffer A.E. (2022). Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron, 110(8), 1340-1357.e7.
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3

Comprehensive Antibody Panel for Cellular Characterization

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The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-pSer13-GFAP (gift from Dr. Masaki Inagaki, clone KT13, IF 1:20), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-Tra-1–60 (Thermo Fisher Scientific, 41–1000, IF 1:300), mouse anti-Tra-1–81 (Thermo Fisher Scientific, 41–1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48–1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat anti mouse and rabbit antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat anti-mouse and rabbit antibodies (Sigma, WB 1:5000).
Battaglia R., Faridounnia M., Beltran A., Robinson J., Kinghorn K., Ezzell J.A., Bharucha-Goebel D., Bonnemann C., Hooper J.E., Opal P., Bouldin T.W., Armao D, & Snider N. (2023). Intermediate filament dysregulation and astrocytopathy in the human disease model of KLHL16 mutation in giant axonal neuropathy (GAN). bioRxiv.
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4

Immunochemical Analysis of Neural Cell Markers

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Immunochemical analysis was carried out as previously described (Yang et al. 2016 (link)). Anti-mouse O4 IgM (50%, v/v) were produced by hybridoma culture. Anti-mouse Olig2 (1:1000; Oasis Biofarm), anti-mouse GFAP (1:1000; Oasis Biofarm), and anti-rabbit neurofilament (NF-1) (1:1000) were purchased from Merck (Darmstadt, Germany). Anti-rat MBP (1:500), anti-rabbit EGFR (1:200) and anti-mouse CC1 (1:500) was obtained from Abcam (Boston, USA). Anti-mouse Nestin (1:1000) and the Alexa-488 or Alexa-594 conjugated secondary antibodies were purchased from Invitrogen (Frederick, USA). The nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) was obtained from Roche (Basel, Switzerland).
Liu X., Li C., Li J., Xie L., Hong Z., Zheng K., Zhao X., Yang A., Xu X., Tao H., Qiu M, & Yang J. (2022). EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes. Molecular Medicine, 28, 50.
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5

Immunochemical Analysis of Cell Markers

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Immunochemical analysis was carried out as previously described [22] . Anti-mouse O4 IgM (50%, v/v) were produced by hybridoma culture. Anti-mouse Olig2 (1:1000), anti-mouse GFAP (1:1000), and antirabbit neuro lament (NF-1) (1:1000) were purchased from Millpore. Anti-rat MBP (1:500) and anti-mouse CC1 (1:500) was obtained from Abcam, and anti-rabbit PDGFRα (1:500) from Santa Cruz. Anti-mouse Nestin (1:1000) and the Alexa-488 or Alexa-594 conjugated secondary antibodies were purchased from Invitrogen. The nucleic acid dye 4',6-diamidino-2-phenylindole (DAPI) was obtained from Roche.
Zhang Y., Xie L., Li J., Li C., Zheng K., Zhao X., Yang A., Xu X., Tao H., Qiu M., & Yang J. (2021). EGF signaling promotes the lineage conversion of astrocytes into oligodendroglias.
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