Anti cd107a apc

Manufactured by Miltenyi Biotec

Anti-CD107a (APC) is a fluorochrome-conjugated antibody that binds to the CD107a (LAMP-1) cell surface protein. CD107a is a marker of lysosomal-associated membrane protein that is expressed on the cell surface during degranulation, a process associated with the activation of cytotoxic cells such as natural killer (NK) cells and cytotoxic T cells.

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Lab products found in correlation

Golgistop, by BD (2 mentions) Navios flow cytometer, by BD (1 mentions) Apc cy7, by BD (1 mentions)

2 protocols using anti cd107a apc

Ruxolitinib Modulates NK Cell Functions

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We incubated purified NK cells (2 × 10⁶ cells/mL) overnight with 100 ng/mL LPS and 10 µg/mL aCpG in the presence or absence of increasing concentrations of ruxolitinib (0.1 µM, 1 µM, 10 µM) at 37°C in complete media. After incubation, we stimulated the NK cells with the tumor cell lines K562, SEM, and MV-4-11 at 1:1 and 1:2 E:T ratios at 37°C for 4 hours in complete media with the specific antibody anti-CD107a (APC, Miltenyi Biotec) and Golgi Stop (BD Biosciences). We then stained the NK cells with 7-aminoactinomycin D (7-AAD) and specific antibodies against the surface receptors CD45 (BV510, Biolegend), CD3 (PCy7, Biolegend), CD16 (APC-Cy7, BD Biosciences), and CD56 (AlexaFluor770, BD Biosciences). We performed a flow cytometry analysis on a Navios Flow Cytometer and employed FlowJo v10.0.7 software (BD Biosciences) for the data analysis.

Mestre-Durán C., Martín-Cortázar C., García-Solís B., Pernas A., Pertíñez L., Galán V., Sisinni L., Clares-Villa L., Navarro-Zapata A., Al-Akioui K., Escudero A., Ferreras C, & Pérez-Martínez A. (2023). Ruxolitinib does not completely abrogate the functional capabilities of TLR4/9 ligand-activated NK cells. Frontiers in Immunology, 13, 1045316.

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NK Cell Degranulation Analysis

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NK cell degranulation was studied as previously described.^{18 (link)} Shortly, CD107a was measured in the membrane using anti-CD107a-APC (Miltenyi) by flow cytometry after 4 hours of incubation of PBMCs with target cells in the presence of BD GolgiStop™ (BD Biosciences). K562 and SH-SY5Y cell lines were used as target cells using a 1:2 PBMC:target ratio. After the incubation, CD3, CD56, CD107a, and PD-1 were extracellularly stained and analyzed by flow cytometry as described above. As a control for specific target cell-mediated degranulation, replicates were made without adding target cells. Results were reported as the percentage of CD107a positive cells in PD-1 positive and negative cells within the CD3⁻ CD56⁺ NK cell population.

Pesini C., Hidalgo S., Arias M.A., Santiago L., Calvo C., Ocariz-Díez M., Isla D., Lanuza P.M., Agustín M.J., Galvez E.M., Ramírez-Labrada A, & Pardo J. (2022). PD-1 is expressed in cytotoxic granules of NK cells and rapidly mobilized to the cell membrane following recognition of tumor cells. Oncoimmunology, 11(1), 2096359.

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