Cfi plan fluor 60 oil

Fluorescent images were acquired using IP Lab software version 3.9.4r4 with a fluorescence colocalization module (Scanalytics, Inc., Fairfax, VA) on a Nikon Diaphot TE300 microscope equipped with CFI Plan Fluor 60× oil, numerical aperture 1.4 and CFI Plan Apo Lambda 100× oil, numerical aperture 1.45 objectives plus 10× projection lens (Nikon, Garden City, NY), SensiCamQE CCD camera (Cooke Corp., Romulus, MI), motorized stage and dual filter wheels (Prior) with single band excitation and emission filters for FITC/TRITC/CY5/DAPI (Chroma, Rockingham, VT). The image size acquired at 60× is 78 μm × 58 μm and the image size acquired at 100× is 41 μm × 30 μm. Calibration bars on images are 10 μm.

The microscope system consists of a Nikon Diaphot TE300 microscope equipped with CFI Plan Fluor 60× oil, numerical aperture (NA) 1.4 and CFI Plan Apo Lambda 100× oil, NA 1.45 objectives, a 10× projection lens (Nikon, Garden City, NY) and a Prior motorized stage. Fluorescent images were obtained with a SensiCamQE CCD camera (Cooke Corp., Romulus, MI) using dual filter wheels (Prior) with single band excitation and emission filters for FITC/TRITC/CY5/DAPI (Chroma, Rockingham, VT). Cell images were processed and analyzed using IP Lab software version 3.9.4r4 with a fluorescence colocalization module (Scanalytics, Inc., Fairfax, VA). Images acquired using the 60× objective have dimensions of 78 µm × 58 µm and images acquired using the 100× objective are 41 µm × 30 µm. Calibration bars on images are 10 µm.