Anti hnrnpa2b1 antibody ab6102

Total proteins were isolated by using RIPA supplemented with protease and phosphatase inhibitor reagents (Thermo-Fisher Scienti c, Waltham, MA, USA). Nuclear and cytoplasm proteins were isolated by PARIS™ Kit (Life) according to the manufacturer's instruction. Protein was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). After transferred proteins to PVDF membranes (Merck Millipore, Billerica, MA, USA), membranes were blocked in 5% skim milk for 1 h at room temperature followed by incubated with primary antibodies overnight at 4 °C. After three washes with TBST, PVDF membranes were incubated with HRP-conjugated goat anti-mouse (#7076, Cell signaling Technology, Danvers, MA, USA) or anti-rabbit (#7074, CST) secondary antibodies for 1 h at room temperature. Anti-GAPDH antibody (#5174), anti-Tubulin antibody (#2146), anti-Lamin B1 antibody (#13435), anti-Snail antibody (#3879), anti-E-cadherin antibody (#3195), anti-N-cadherin antibody (#13116), anti-ERK1/2 antibody (#9102), anti-Phospho-ERK1/2 antibody (#9101), anti-Raf-1 antibody (#9422) were from Cell Signaling Technology; anti-hnRNPA2B1 antibody (ab6102) was from Abcam (Cambridge, MA, USA).