Irbp1 20 gpthlfqpslvldmakvlld

Induction of RD: RD was induced in mice by subretinal injection of sodium hyaluronate detailed in our recent publication (Augustine et al. 2019) . Mouse eyes were collected 7 days after induction of RD and processed for immunofluorescence staining.
Induction of EAU: EAU was induced in C57BL/6J mice by immunizing the animals with 500 mg of IRBP1-20 (GPTHLFQPSLVLDMAKVLLD, GL Biochem,Shanghai, China) using the protocol reported in our previous publications (Xu et al. 2008; Chen et al. 2018) . Mouse eyes were collected 25 days after induction of EAU and processed for immunofluorescence staining.

Eight-to 12-weekeold C57BL/6J, SOCS3 fl/fl and LysM Cre/þ SOCS3 fl/fl mice (both in C57BL/6J background) were used in the study. The LysM Cre/þ SOCS3 fl/fl mice were obtained by crossbreeding the SOCS3 fl/fl mice and the LysM-Cre mice. The absence of SOCS3 in myeloid cells in the LysM Cre/þ SOCS3 fl/fl mice was confirmed by Western blotting of peritoneal macrophages. The SOCS3 fl/fl mice were used as controls in some experiments. All mice were maintained in the Biological Research Unit at Queen's University Belfast and exposed to a 12-hour light/night cycle with free access to water and food. All procedures were conducted under the regulation of the UK Home Office Animals (Scientific Procedures) Act 1986 and approved by the Animal Welfare & Ethical Review Body of Queen's University Belfast.
EAU was induced in mice using a protocol described previously. 22, 23 Briefly, mice were immunized with 500 mg of IRBP 1-20 (GPTHLFQPSLVLDMAKVLLD, GL Biochem, Shanghai, China), emulsified 1:1 in complete Freund's adjuvant (Difco Laboratories, Detroit, MI), and supplemented with 2.5 mg/mL of Mycobacterium tuberculosis H37Ra (Difco Laboratories). An additional 1 mg of Bordetella pertussis toxin (Tocris Bioscience, Bristol, UK) was administered intraperitoneally immediately after immunization injection.