Adult mice were deeply anesthetized with 2% avertin and perfused with 0.1 M PBS followed by 4% paraformaldehyde. Brains were postfixed in 4% paraformaldehyde at 4°C for 24 hours and 30% sucrose at 4°C for 48 hours. Frozen brains were then cut into 30 μm coronal cryosections. Sections were blocked in 0.1 M PBS containing 0.3% Triton X-100 (Sigma) and 2% Donkey Serum (GeneTex) for 30 min at room temperature. Primary antibody was applied at the appropriate dilution and incubated overnight at 4°C. Incubated sections were washed three times with 0.1 M PBS and incubated in secondary antibody for two hours. After three rinses in 0.1 M PBS and DAPI staining at 1:1000 (Pierce), the sections were mounted on polysine microscopic glass slides (Thermo Scientific). Images were scanned with the Axio Scan Z1 automated slide scanner (Zeiss) using ZEN 2 (blue edition) slidescan software (Zeiss).
Axio scan z1 automated slide scanner
Manufactured by Zeiss
The Axio Scan Z1 is an automated slide scanner designed for high-throughput digital microscopy. The device captures high-resolution digital images of microscope slides, enabling efficient digitization and archiving of samples.
Automatically generated - may contain errors
2 protocols using axio scan z1 automated slide scanner
1
Anatomical Characterization of Mouse Brain
2
Anatomical Characterization of Mouse Brain
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Adult mice were deeply anesthetized with 2% avertin and perfused with 0.1 M PBS followed by 4% paraformaldehyde. Brains were postfixed in 4% paraformaldehyde at 4°C for 24 hours and 30% sucrose at 4°C for 48 hours. Frozen brains were then cut into 30 μm coronal cryosections. Sections were blocked in 0.1 M PBS containing 0.3% Triton X-100 (Sigma) and 2% Donkey Serum (GeneTex) for 30 min at room temperature. Primary antibody was applied at the appropriate dilution and incubated overnight at 4°C. Incubated sections were washed three times with 0.1 M PBS and incubated in secondary antibody for two hours. After three rinses in 0.1 M PBS and DAPI staining at 1:1000 (Pierce), the sections were mounted on polysine microscopic glass slides (Thermo Scientific). Images were scanned with the Axio Scan Z1 automated slide scanner (Zeiss) using ZEN 2 (blue edition) slidescan software (Zeiss).
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