The 5-methylcytosine level in the DNA of LPLs was measured using a MethylFlash Global DNA
Methylation (5-mC) ELISA Easy Kit (Epigentek Group Inc., Farmingdale, NY, USA) according
to the manufacturer’s protocol. The absorbance of each well at 450 nm was measured using a
Varioskan Flash microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), and the
5-mC concentration was calculated using a standard curve.
AIZAWA S., UEBANSO T., SHIMOHATA T., MAWATARI K, & TAKAHASHI A. (2023). Effects of the loss of maternal gut microbiota before pregnancy on gut microbiota, food allergy susceptibility, and epigenetic modification on subsequent generations. Bioscience of Microbiota, Food and Health, 42(3), 203-212.