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Avarioskan flash microplate reader

Manufactured by Thermo Fisher Scientific
Sourced in United States

The VarioskanTM Flash microplate reader is a versatile instrument designed for a wide range of photometric measurement applications. It is capable of performing absorbance, fluorescence, and luminescence measurements in microplates.

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2 protocols using avarioskan flash microplate reader

1

Quantifying Global DNA Methylation

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The 5-methylcytosine level in the DNA of LPLs was measured using a MethylFlash Global DNA
Methylation (5-mC) ELISA Easy Kit (Epigentek Group Inc., Farmingdale, NY, USA) according
to the manufacturer’s protocol. The absorbance of each well at 450 nm was measured using a
Varioskan Flash microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), and the
5-mC concentration was calculated using a standard curve.
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2

Bacterial Growth Curve Measurement

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OD600 measurements were determined on a
Varioskan Flash microplate reader (Thermo, MA). Bacteria cells (200
μL/well) were cultured in flat-bottomed 96-well plates (3599,
Corning, NY) at 37 °C and 200 rpm. OD600 was measured
in triplicate every 2 h from the initial bacteria culture. Thus, the
growth curves of the three strains were determined from the OD600 measurements over a 24 h period.57 (link),58 (link)
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