For immunofluorescence staining, hiPSC colonies and RPCs were fixed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature. hiPSC colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scientific, 10 μg/mL), and anti-Sinaptopodin (Thermo Scientific, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scientific, 10 μg/mL), and Sinaptopodin (Thermo Scientific, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scientific, Waltham, MA, USA).
Anti oct4a rabbit mab
Manufactured by Cell Signaling Technology
Anti-OCT4A Rabbit mAb is a monoclonal antibody that specifically recognizes the OCT4A isoform of the OCT4 transcription factor. OCT4A is a key regulator of pluripotency and plays a critical role in the maintenance of embryonic stem cell identity.
Automatically generated - may contain errors
Lab products found in correlation
Anti nanog, by Cell Signaling Technology (3 mentions)
Anti pax2, by Abcam (3 mentions)
Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (3 mentions)
Vectashield antifade mounting medium, by Vector Laboratories (3 mentions)
Anti nephrin, by Thermo Fisher Scientific (3 mentions)
Nephrin, by Thermo Fisher Scientific (3 mentions)
Anti sinaptopodin, by Thermo Fisher Scientific (2 mentions)
Sinaptopodin, by Thermo Fisher Scientific (2 mentions)
Evos fl imaging system, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti oct4a rabbit mab
1
Immunofluorescence Staining of hiPSC and RPC
2
Immunofluorescence Staining of hiPSCs and RPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immuno uorescence staining, hiPSCs colonies and RPCs were xed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1h at room temperature. hiPSCs colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scienti c, 10 μg/mL), and anti-Sinaptopodin (Thermo Scienti c, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scienti c, 10 μg/mL), and Sinaptopodin (Thermo Scienti c, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scienti c, Waltham, MA, USA).
3
Immunofluorescence Staining of hiPSCs and RPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immuno uorescence staining, hiPSCs colonies and RPCs were xed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1h at room temperature. hiPSCs colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scienti c, 10 μg/mL), and anti-Sinaptopodin (Thermo Scienti c, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scienti c, 10 μg/mL), and Sinaptopodin (Thermo Scienti c, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scienti c, Waltham, MA, USA).
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