The content of calcium, iron, magnesium, manganese, potassium, sodium, phosphorus, zinc, and total sulfur was analyzed for all samples following the Standard Methods for the Examination of Water and Waste Water (SMWW) methodology described by APHA (ed 23 2017). The pH and conductivity of the water samples were measured by using a pH meter (Mettler Toledo Seven compact duo S213, Columbus, Ohio, USA) and a conductivity meter (Mettler Toledo Seven compact duo S213, Columbus, Ohio. USA) respectively. The probe was calibrated using a standard solution with a known conductivity. The analyses of metals were carried out based on APHA standards (method 2320B, Waltham, MA, USA) using Inductively Coupled Plasma (ICP-OES, Perkin Elmer Optima 8300).
Rojas-Gätjens D., Arce-Rodríguez A., Puente-Sánchez F., Avendaño R., Libby E., Mora-Amador R., Rojas-Jimenez K., Fuentes-Schweizer P., Pieper D.H, & Chavarría M. (2021). Temperature and elemental sulfur shape microbial communities in two extremely acidic aquatic volcanic environments. Extremophiles : life under extreme conditions, 25(1).
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