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3 protocols using pure white crystalline cholesterol powder

1

Cholesterol and Biochemical Analysis Protocol

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Pure white crystalline cholesterol powder and saline solutions were purchased from SIGMA Chemical Co., (USA). Casein, cellulose, choline chloride powder, and DL-methionine powder, were obtained from Morgan Co. Cairo, Egypt. Chemical kits used in this study (TC, TG, HDL-c, ALT, AST, ALP, urea, creatinine, serum protein) were obtained from Al-Gomhoria Company for Drugs, Chemical and Medical Instruments, Cairo, Egypt. Folin-Ciocalteu reagent and standard substances including gallic acid, caffeic acid, oleuropein, protocatechuic acid and pcoumaric acid were purchased from Sigma Chemical Company (St. Louis, USA), synergic acid, ferulic acid, rutin and Quercetin from SIGMA Chemical Co., (USA). All reagents and standards were prepared using Milli-Q deionized water (Millipore, Bedford, USA). All other chemicals and reagents were of analytical reagent grade and purchased from Al-Ghomhoria Company, Egypt.
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2

Apricot, Plum Kernels Characterization

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Commercially dried and ground apricot (Prunus armeniaca, L), plum (Prunus domestica, L) kernels and their mixture were obtained from local market in 2019 from local market at Menoufia Governorate, Egypt. Pure white crystalline cholesterol powder and saline solutions were purchased from SIGMA Chemical Co., (USA). Casein, cellulose, choline chloride powder, and DL methionine powder, were obtained from Morgan Co. Cairo, Egypt. Chemical kits used in this study (TC, TG, HDL-c, ALT, AST, ALP, bilirubin, urea, creatinine, albumin) were obtained from Al-Gomhoria Company for Drugs, Chemical and Medical Instruments, Cairo, Egypt. While, malondialdehyde kits was obtained from SIGMA Chemical Co., Cairo, Egypt.
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3

Dietary Supplementation of Mango and Avocado Seeds

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Commercially dried and ground mango (Mangiferaindica, L), avocado (Perseaamericana, L) seeds and their mixture were obtained from local market in August 2018 from local market at Menoufia Governorate, Egypt. Chemicals pure white crystalline cholesterol powder and saline solutions were purchased from SIGMA Chemical Co., (USA). Casein, cellulose, choline chloride powder, and DL methionine powder, were obtained from Morgan Co. Cairo, Egypt. Chemical kits used in this study (TC, TG, HDL-c, ALT, AST, ALP, bilirubin, urea, creatinine, albumin) were obtained from Al-Gomhoria Company for Drugs, Chemical and Medical Instruments, Cairo, Egypt.
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