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Itlc sg paper

Manufactured by Pall Corporation

ITLC-SG paper is a laboratory product manufactured by Pall Corporation. It is an inert thin-layer chromatography (TLC) support material used for analytical and preparative separations.

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2 protocols using itlc sg paper

1

ITLC Analysis of [177Lu]-DOTATATE Purity

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The ITLC test is used to determine the percentage of [177Lu]-DOTATATE and 177Lu impurities in the final product. The ITLC paper strips are counted in a laboratory gamma-counter (PerkinElmer Wizard2 (link)® Automated Gamma Counter, PerkinElmer Downers Grove, IL, USA) and results entered into a spreadsheet (Microsoft Excel Version 2010) for final calculation and analysis.
For the determination of percentage [177Lu]-DOTATATE and 177Lu impurities, 0.1 mol/L citrate buffer (pH= 5) with ITLC-SG paper (Pall Corporation, East Hills, New York); Rf (free 177Lu) = 0.8–1.0, Rf (177Lu peptide) = 0.0–0.3 is used.
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2

Ga-68 DOTATATE Purity Quantification

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The ITLC test is used to determine the percentage of Ga-68 DOTATATE, Ga-68 impurities, and Ga-68 colloid in the final product. The ITLC paper strips are counted in a laboratory gamma-counter (PerkinElmer Wizard2® Automated Gamma Counter, PerkinElmer Downers Grove, IL USA) and results entered into a spread sheet (Microsoft Excel Version 2010) for final calculation and analysis.
For the determination of percentage Ga-68 DOTATATE and Ga-68 impurities, 0.1 mol/L citrate buffer (pH=5) with ITLC-SG paper (Pall Corporation, East Hills, New York) is used; free Ga-68 (Rf=0.8–1), Ga-68 peptide (Rf=0.0–0.3) and Ga-68 colloid (Rf= 0.0-0.2).
For the determination of percentage Ga-68 colloid, 1 mol/L ammonium acetate / methanol (1 : 1) / ITLC-SG (Pall Corporation) is used; Ga-68 colloid (Rf= 0.0-0.2), Ga-68 peptide (Rf= 0.8-1) and free Ga-68 (Rf=0.8–1).
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