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Propidium iodide

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Propidium iodide is a fluorescent dye commonly used in life science research. It is a nucleic acid intercalator that binds to DNA, emitting a red fluorescence when excited by a laser or light source. Propidium iodide is often used to stain and identify dead cells in a sample.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (3 mentions) Propidium iodide, by Biotium (2 mentions) Cellometer spectrum, by Revvity (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Cellometer, by Revvity (1 mentions) Annexin 5 fitc, by Revvity (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Celigo imaging cytometer, by Revvity (1 mentions) Hoechst 33342, by Revvity (1 mentions) Calcein acetoxymethyl, by Thermo Fisher Scientific (1 mentions) Collagenase from clostridium histolyticum, by Merck Group (1 mentions) Cellometer k2, by Revvity (1 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Doxorubicin dox, by Selleck Chemicals (1 mentions) Complete mammocult medium, by STEMCELL (1 mentions) Slide a lyzer dialysis cassette, by Thermo Fisher Scientific (1 mentions) Leptin, by R&D Systems (1 mentions) Accutase, by Innovative Cell Technologies (1 mentions) Acridine orange, by Revvity (1 mentions) Papain dissociation kit, by Worthington (1 mentions)

Close spelling variants of this product

Acridine Orange/Propidium Iodide ViaStain acridine orange/propidium iodide solution Acridine orange/propidium iodide (1:1) solution

7 protocols using propidium iodide

1

Apoptosis Analysis of Drug-Treated Cells

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Apoptotic properties of the treated or untreated cells were analyzed using Cellometer from Nexcelom Bioscience according to the manufacturer’s instructions. HL-60 cells were treated with cisplatin (1, 2, or 3 µM), Arsenic Trioxide (ATO) (20 µM), or left untreated. After 24, 48, 72, or 96 hour incubation, the cells were washed with PBS and the pellets were dissolved in Annexin V binding buffer followed by incubation with Annexin V-FITC and propidium iodide (PI; Nexcelom Bioscience) according to the manufacturer’s protocol. The stained cells were analyzed using Cello meter and Vision CBA software from Nexcelom Bioscience.
Velma V., Dasari S.R, & Tchounwou P.B. (2016). Low Doses of Cisplatin Induce Gene Alterations, Cell Cycle Arrest, and Apoptosis in Human Promyelocytic Leukemia Cells. Biomarker Insights, 11, 113-121.
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2

Live Cell Quantification by Imaging

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The number of live cells was determined by co-staining the cells with Hoechst 33342 (to identify the number of nuclei, Life Technologies) and propidium iodide (to identify dead cells, Thermo Fisher Scientific). The number of live cells was determined by subtracting the number of propidium iodide-positive cells from the Hoechst 33342-positive cells identified using a Celigo imaging cytometer (Nexcelom).
Gheller B.J., Blum J.E., Lim E.W., Handzlik M.K., Hannah Fong E.H., Ko A.C., Khanna S., Gheller M.E., Bender E.L., Alexander M.S., Stover P.J., Field M.S., Cosgrove B.D., Metallo C.M, & Thalacker-Mercer A.E. (2020). Extracellular serine and glycine are required for mouse and human skeletal muscle stem and progenitor cell function. Molecular Metabolism, 43, 101106.
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3

Quantifying Cell Viability via Propidium Iodide

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In 6-well plates, 150,000 cells were plated and allowed to attach overnight. Cells were treated with vehicle and 10 μM enzalutamide for 72 h. Propidium iodide (#40017, Biotium) was added to each well at a concentration of 1 μg/mL and incubated for 15 min at 37°C. Supernatant was collected as well as trypsinized cells. Propidium iodide-positive cells (non-viable cells) were counted using Cellometer Spectrum (Nexcelom Bioscience; Lawrence, MA).
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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4

Quantification of Viable Adipocytes

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Each processed fat graft (10 mL) was digested for 1 hour at 37°C with 250 U/mL collagenase from Clostridium histolyticum (Sigma-Aldrich, St. Louis, Mo.) at a 1:4 fat graft to collagenase solution ratio. The adipocytes were isolated using centrifugation and stained for 30–60 minutes at 37°C with a live-dead cell-staining solution containing calcein acetoxymethyl (Invitrogen, Waltham, Mass.) and propidium iodide (Nexcelom Bioscience, Lawrence, Mass.) in PBS. The stained adipocyte suspensions were diluted and placed onto SD300 slides (Nexcelom Bioscience), and the adipocytes were counted with a Cellometer K2 (Nexcelom Bioscience).
Gabriel A., Kabaria N., Fang C.H., Lombardi J.A., Stec E., Huang L.T., Li H, & Sandor M. (2024). In Vitro Characterization of Fat Grafts Processed Using the REVOLVE ENVI System versus Decantation. Plastic and Reconstructive Surgery Global Open, 12(2), e5615.
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5

Quantifying Cell Viability via Propidium Iodide

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In 6-well plates, 150,000 cells were plated and allowed to attach overnight. Cells were treated with vehicle and 10 μM enzalutamide for 72 h. Propidium iodide (#40017, Biotium) was added to each well at a concentration of 1 μg/mL and incubated for 15 min at 37°C. Supernatant was collected as well as trypsinized cells. Propidium iodide-positive cells (non-viable cells) were counted using Cellometer Spectrum (Nexcelom Bioscience; Lawrence, MA).
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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6

Nanoparticle-Based Breast Cancer Treatment

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IONPs were obtained from Ocean Nanotech San Diego, CA. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC), Sulfo-NHS, and other chemicals were purchased from Sigma Aldrich St. Louis, MO. Ob-R (sc-8325), Cyclin D1 (sc-246), pSTAT3 (sc-8059), STAT3 (sc-8019) antibodies were purchased from Santa Cruz Biotechnology Santa Cruz, CA. Anti-rabbit and anti-mouse conjugated to horseradish peroxidase were obtained from Bio-Rad Laboratories Hercules, CA. Dulbecco’s Modified Eagles Medium (DMEM), Iscove’s Modified Dulbecco’s Medium (IMEM), Protease and Phosphatase Inhibitor cocktails, Penicillin/Streptomycin, Slide-a-lyzer dialysis cassette, and Western blotting chemiluminescence substrate were purchased from Thermo Fisher Scientific Rockford, IL. Mammocult complete medium was obtained from Stem Cell Technologies Vancouver, BC. Fetal bovine serum was obtained from Med Supply Partners Atlanta, GA. Leptin was purchased from R and D Systems Minneapolis, MN. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit was purchased from Molecular Probes Eugene, OR. Annexin V/fluorescein Isothiocyanate (FITC) and propidium iodide (PI) were obtained from Nexcelom Bioscience Boston, MA. Cisplatin (Cis) was purchased from Millipore Billerica, MA. Cyclophosphamide (CTX), paclitaxel (PTX), and doxorubicin (Dox) were obtained from SelleckChem Houston, TX.
Harmon T., Harbuzariu A., Lanier V., Lipsey C.C., Kirlin W., Yang L, & Gonzalez-Perez R.R. (2017). Nanoparticle-linked antagonist for leptin signaling inhibition in breast cancer. World Journal of Clinical Oncology, 8(1), 54-66.
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7

Xenograft Survival Analysis Protocol

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All animal experiments were done in accordance with protocols approved by our Institutional Animal Care and Use Committee and following NIH guidelines for animal welfare. Cells were dissociated with Accutase (Innovative Cell Technologies) and the number of live cells was counted by Acridine Orange (AO) and Propidium Iodide (PI) staining (Nexcelom). A papain dissociation kit (Worthington Biochemical) was used for the dissociation of spheroids. Cells were resuspended in PBS and the indicated number of cells was stereotactically transplanted into the brain of NOD/SCID mice. Male and female mice were evenly assigned to each group. Coordinates of the injection site were 2 mm lateral and 2 mm posterior to bregma and 2 mm deep from the head skull. Mice were monitored for up to 6 months. Survival of mice was evaluated by Kaplan-Meier analysis and p-values were calculated using a log-rank test. For in vivo imaging, cells were lentivirally labeled with luciferase and luminescence was measured using IVIS Spectrum In Vivo Imaging System.
Funato K., Smith R.C., Saito Y, & Tabar V. (2021). Dissecting impact of regional identity and oncogenic role of human-specific NOTCH2NL in an hESC model of H3.3G34R mutant glioma. Cell stem cell, 28(5), 894-905.e7.
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