1,2-dipentadecanoyl-
sn-glycero-3-phosphoethanolamine (PE 15:0/15:0), 1-palmitoyl-2-oleoyl-
sn-glycero-3-phosphoethanolamine (PE 16:0/18:1(9Z)), 1-(1Z-octadecenyl)-2-arachidonoyl-
sn-glycero-3-phosphoethanolamine (PE P-18:0/20:4(5Z,8Z,11Z,14Z)), 1-tridecanoyl-
sn-glycero-3-phosphoethanolamine (LPE 13:0/0:0) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). All of them were >99% pure, except LPE 13:0/0:0 may contain up to 10% of sn-2 isomer. [U-
13C
6]-
D-glucose (99%) was from Cambridge Isotope Laboratories (Tewksbury, MA, USA).
D-glucose and all solvents including methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), chloroform (CHCl
3), and water (H
2O) of LC-MS grade and HPLC grade were purchased from Fisher Scientific (Pittsburgh, PA, USA).
Pooled human plasma collected from healthy people with
K2-EDTA as anticoagulant was purchased from BioIVT (Westbury, NY, Lot#: BRH1524451). The standard reference material—metabolites in frozen human plasma (NIST SRM-1950)—was purchased from the NIST (Gaithersburg, MD).
He X., Li Z, & Zhang Q. (2020). A UPLC-MRM-MS method for comprehensive profiling of Amadori compound-modified phosphatidylethanolamines in human plasma. Analytical and bioanalytical chemistry, 413(2), 431-443.