HSCs were purchased from ScienCell Research Laboratories (San Diego, CA). Cells were activated after they were cultured for 7 days on plastic dishes. Sorafenib and Selumetinib (AZD6244) was obtained from LC Laboratories (Woburn, MA). For in vivo liver metastasis studies, the Pancreatic ductal adenocarcinoma (PDAC) cells AK4.4 were stably transduced with Gluc/GFP gene by using a retroviral vector. Carbon tetrachloride (CCl4), olive oil, coumarin 6, dimethyl sulfoxide (DMSO), D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) and paraformaldehyde were purchased from Sigma-Aldrich (St, Louis, MO). 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG-Maleimide) were obtained from Avanti Polar Lipids (Alabaster, AL). poly(lactic-co-glycolic acid) (PLGA) (50/50, inherent viscosity: 0.17 dL/g) was ordered from Green Square Materials Incorporation (Taoyuan, Taiwan). Tris(2-carboxyethyl)phosphine (TCEP) disulfide reducing gels were purchased from Thermo Fisher. CTCE9908 peptide (sequence: Lys-Gly-Val-Ser-Leu-Ser-Tyr-Arg-Cys-Arg-Tyr-Ser-Leu-Ser-Val-Gly-Lys) and scramble peptide (Leu-Tyr-Ser-Val-Lys-Arg-Ser-Gly-Cys-Gly-Ser-Arg-Lys-Val-Ser-Tyr-Leu) were synthesized by Kelowna International Scientific Incorporation.
Selumetinib
Manufactured by LC Laboratories
Sourced in United States
Selumetinib is a chemical compound used as a laboratory research tool. It functions as a selective inhibitor of the enzyme MEK.
Automatically generated - may contain errors
Lab products found in correlation
Sorafenib, by LC Laboratories (2 mentions)
Crizotinib, by LC Laboratories (2 mentions)
Sunitinib, by LC Laboratories (2 mentions)
D α tocopherol polyethylene glycol 1000 succinate tpgs, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Caspase, by Cell Signaling Technology (1 mentions)
Cleaved caspase, by Cell Signaling Technology (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ac devd afc caspase 3 fluorogenic substrate, by BD (1 mentions)
Fxcycle pi rnase, by Thermo Fisher Scientific (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Annexin 5 apc, by BD (1 mentions)
Entinostat, by Selleck Chemicals (1 mentions)
5 protocols using selumetinib
1
Optimizing Liver Metastasis Treatment
2
Kinase Inhibitor Combination Treatment
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Cells were treated with AKTi (Tocris), selumetinib (LC Laboratories), or a combination of both drugs for 24 h to final concentrations of 10 and 50 µM, respectively. Both drugs were dissolved in DMSO. 8-azaadenosine (8-aza) (AKOS030530872, Akos Consulting & Solutions GmbH) was used to inhibit RNA editing. 8-aza dissolved in water was added to the cell culture medium at the indicated final concentrations.
3
Crizotinib and Selumetinib Kinase Inhibition
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Crizotinib and selumetinib were purchased from LC laboratories (Woburn, Massachusetts, USA). Bovine serum albumin (BSA), Foetal bovine serum (FBS), Rosswell park memorial institute medium (RPMI), penicillin/streptomycin were purchased from Life Technologies (Auckland, New Zealand). Precision plus protein kaleidoscope, acrylamide (1:30) were obtained from Bio-Rad Laboratories (Hercules CA, USA). CL-XPosure film, supersignal west pico were obtained from Thermofisher (Auckland, New Zealand). Propidium iodide was purchased from Sigma- Aldrich (St louis, MO, USA). FxCycle PI/RNase was from Life technologies (California, USA). Annexin V-APC and Ac-DEVD-AFC caspase-3 fluorogenic substrate was purchased from BD Biosciences (New Jersey, USA).
Antibodies against ALK(D5F3), phosphorylated-ALK (Tyr1604), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), Bim, Bcl2, caspase, cleaved caspase, PARP, cleaved PARP, cyclinD1, p27 were purchased from Cell Signaling Technology (Danvers, MA, USA). Erk1/2 and β-tubulin antibodies were purchased from Sigma-Aldrich (St louis, MO, USA). HRP-conjugated goat anti-rabbit and HRP-conjugated goat anti-mouse were obtained from Calbiochem (San Diego, CA, US).
Antibodies against ALK(D5F3), phosphorylated-ALK (Tyr1604), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), Bim, Bcl2, caspase, cleaved caspase, PARP, cleaved PARP, cyclinD1, p27 were purchased from Cell Signaling Technology (Danvers, MA, USA). Erk1/2 and β-tubulin antibodies were purchased from Sigma-Aldrich (St louis, MO, USA). HRP-conjugated goat anti-rabbit and HRP-conjugated goat anti-mouse were obtained from Calbiochem (San Diego, CA, US).
4
Acquisition and Preparation of Pharmacological Agents
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Cetuximab was purchased from Merck (Dietikon, Switzerland). Gefitinib was purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands); Y27632 was purchased from Tocris Bioscience (Bristol, UK). Sunitinib, selumetinib, and vemurafenib were purchased from LC Laboratories (Woburn, MA, USA). C646 was purchased from Axon Medchem (Groningen, the Netherlands). SAHA and entinostat were purchased from Selleckchem (Munich, Germany). Cis-Diamineplatinum (II) dichloride and staurosporine were purchased from Sigma (Aldrich, Nederland). Doxrubicin was purchased from Teva Pharmaceuticals. VI83, which inhibits PDGFRß, was a kind gift from Vichem Laboratories (Budapest, Hungary). CXCR7 inhibitor was a kind gift from Professor Rob Leurs and Professor Martine J. Smit (Vrije Universiteit Amsterdam, Amsterdam, The Netherlands). All drugs were diluted and aliquoted in dimethyl sulfoxide (DMSO), stored at −20 °C.
5
Anticancer Drug Signaling Pathway Analysis
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Sorafenib, nilotinib, sunitinib, imatinib, gefitinib, lapatinib, dasatinib, PD-173074, selumetinib, crizotinib, tofacitinib and pazopanib were purchased from LC Laboratories (Woburn, Massachusetts, USA). Roswell Park Memorial Institute Medium (RPMI) and Geltrex basement membrane was purchased from Life Technologies (North Shore City, New Zealand). Met, P-Met (Y1234/Y1235), SRC, P-SRC (Y416), AKT, P-ATK (S473), cyclin D1, cleaved caspase 3, α-tubulin and EGFR antibodies were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). β-Tubulin was purchased from Sigma-Aldrich (Auckland, New Zealand).
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