Hut78

The Balb/c mice used in this study were purchased from Guangdong Medical Laboratory Animal Center (Guangdong, China) and were maintained under pathogen-free conditions in the animal facility. All procedures involving mice were approved by the Institutional Animal Care and Use Committee of Chinese PLA General Hospital and General Hospital of Shenzhen University. All experiments were conducted in accordance with the US Department of Health and Human Services Guide for the Care and Use of Laboratory Animals and institutional guidelines. WEHI3 (mouse leukemia cell line), U937 (human myeloid leukaemia cell line), Raji (human B lymphoblastoid cell line), Z-138 (human B lymphoblastoid cell line), Hut-78 (cutaneous T cell lymphoma cell line), Jurkat (human T lymphocyte cell line), Molt-4 (human T lymphoblast cell line), Kasumi-1 (human acute myeloid leukemia cell line), NB-4 (acute promyelocytic leukemia cell line), THP-1 (human monocytic cell line), and K562 (human immortalized myelogenous leukemia cell line) cells were purchased from ATCC (Manassas, VA, USA) and cultured according to the guidelines provided by ATCC.

Karpas 299 (ALK-positive anaplastic large cell lymphoma, a PTCL subtype) and HuT78 (CTCL) cell lines were obtained from ATCC, Gaithersburg, MD, USA. Mac-1 (developed by M.E.K.) was derived from circulating CTCL cells from a patient with multiple T-cell neoplasms [34 (link)]. Karpas 299 and Mac-1 were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Clontech Laboratories, Inc, Mountain View, CA, USA) and 1% penicillin/streptomycin (Gibco, Grand Island, NY, USA). HuT78 was maintained in Iscove's Modified Dulbecco's Medium (Gibco, Grand Island, NY, USA) containing 15% FBS and 1% penicillin/streptomycin. All cell lines were electroporated with 300V for 10 msec in antibiotic-free medium using the ECM 830 electroporation system (BTX Harvard Apparatus).