Pump 33 dds

Manufactured by Harvard Apparatus

Sourced in United Kingdom, United States

The Pump 33 DDS is a programmable syringe pump that can precisely control the flow rate and volume of fluids. It features a digital display and user-friendly interface for setting parameters. The pump can accommodate a variety of syringe sizes and supports both infusion and withdrawal modes.

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Lab products found in correlation

Fm1 43 dye, by Thermo Fisher Scientific (1 mentions) Sucrose, by Merck Group (1 mentions) Paraformaldehyde solution, by Electron Microscopy Sciences (1 mentions) Dextrose, by Merck Group (1 mentions) Qx200 droplet generation oil, by Bio-Rad (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cerulein, by Bio-Techne (1 mentions) Aca1300 200uc, by Basler (1 mentions)

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Pump 33 (6 citations)

6 protocols using pump 33 dds

Droplet Generation and Imaging Protocol

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QX200 Droplet Generation Oil was purchased from Bio-Rad (Hercules, CA). High voltage power supplies were purchased from Bertan High Voltage Corp. (Hicksville, NY). A 488 nm laser with a CDRH LP 1041441 AG power supply was purchased from Coherent Inc. (Santa Clara, CA). Avalanche photodiodes (SPCM-AQRH-11-FC) were purchased from Excelitas Technologies (Waltham, MA). A Pump 33 DDS was purchased from Harvard Apparatus (Holliston, MA). Trihloro(1H,1H,2H,2H-perfluorooctyl)silane (97%), dimethyl sulfoxide (DMSO), Calbiochem Tween 20, sucrose, and dextrose were purchased from Sigma-Aldrich (St. Louis, MO). FM 1-43 dye was purchased from Thermo Fisher Scientific; 100 μg of FM 1-43 was dissolved in 46 μl of DMSO. Buffer solution was composed of 8.0% sucrose, 0.3% dextrose, and 0.1% BSA in 1 mM Tris pH 8.3. Paraformaldehyde solution (4%) was purchased from Electron Microscopy Sciences (Hatfield, PA). Costar 3595 96-well (~6.9 mm inlet DI) TC-Treated micro-plates was purchased from Thermo Fisher Scientific.

Qin Y., Wu L., Wang J., Han R., Shen J., Wang J., Xu S., Paguirigan A.L., Smith J.L., Radich J.P, & Chiu D.T. (2019). A Fluorescence-Activated Single-Droplet Dispenser for High Accuracy Single-Droplet and Single-Cell Sorting and Dispensing. Analytical chemistry, 91(10), 6815-6819.

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Liquid Flow Cell Spectroscopy for Protein Analysis

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The sample cell and the reference cell were liquid flow cells (GS20572, Specac) consisting of two 3 mm thick CaF₂ windows and a lead spacer with a nominal path length of 26 μm. The reference cell was filled with distilled water and sealed during the measurement. The sample cell was filled with either an analyte solution or a solvent. A syringe pump (Pump 33 DDS, Harvard Apparatus) was used to replace liquids in the sample cell. A volume of 600 µL liquid was infused with a flow rate of 50 µL/min, and a single liquid replacement took 12 min. The entire optical system was enclosed and continuously purged with dry air. Bovine serum albumin (BSA, > 96%, Sigma-Aldrich) was used as received. A stock solution of BSA in distilled water was prepared at 10 mg/mL and diluted to desired concentrations in the range of 1 down to 0.02 mg/mL.

Kim S.M., Chang Y.R, & Lee Y.J. (2023). Single-detector double-beam modulation for high-sensitivity infrared spectroscopy. Scientific Reports, 13, 18231.

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Microfluidic Spiral Synthesis of Hollow Silica Spheres

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Details related to the chemical reagents used are presented in the text of Supporting Information. All experiments were conducted at room temperature unless otherwise indicated. The 2-run microfluidic spiral channel with two inlets and one outlet was fabricated using polydimethylsiloxane (PDMS) through soft lithography. Microchannels were formed by bonding the PDMS replica to a standard glass slide after oxygen plasma treatment. The spiral-shaped microchannel is made of three arcs with the diameters of 7.69, 13.8, and 22.2 mm, and with the central angles of 180°, 180°, and 225°, respectively. The height and width of the microchannel are 50 and 500 μm, respectively. The synthesis of HSS was realized simply with one inlet containing CTAB (13.7 mM) in diluted ammonia (0.7 M) and the other containing TMB (0.6 M) in diluted TEOS (0.3 M). The two inlet flows were pumped (Pump 33 DDS, Harvard Apparatus) into the spiral microchannel at a same flow rate of 1 mL/min. The as-synthesized products were collected at the outlet. Details related to the simulation, additional characterization, and other multifunctional materials synthesis results are presented as Supporting Information.

Hao N., Nie Y., Xu Z., Closson A.B., Usherwood T, & J. Zhang J.X. (2019). Microfluidic continuous flow synthesis of functional hollow spherical silica with hierarchical sponge-like large porous shell. Chemical engineering journal (Lausanne, Switzerland : 1996), 366, 433-438.

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Induction of Experimental Pancreatitis in Mice

Cited 3 times

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These protocols were performed by following our previously publications.¹³ (link)^,⁴⁹ (link)^,⁷³ (link)^,⁷⁴ (link) Mice received four, seven, or 12 intraperitoneal injections of cerulein (50 μg/kg; catalog number 6264, Tocris Bioscience; Ellisville, MO) at hourly intervals to induce hyperstimulation AP (CER-AP), while control mice received saline injections of the same volume at the same intervals.¹³ (link)^,⁴⁹ (link)^,⁷³ (link)^,⁷⁴ (link) Taurolithocholic acid 3-sulfate disodium (TLCS; 3 or 5 mM; T0512, Sigma-Aldrich, St. Louis, MO) was retrogradely infused (5 μL/min for 10 min) into the pancreatic duct via a mini-pump (Pump33DDS, Harvard Apparatus; Boston, MA) to induce AP (TLCS-AP).¹³ (link)^,⁴⁹ (link)^,⁷³ (link) Mice were anesthetized with isoflurane immediately prior and during the infusion TLCS or saline.

Du W., Liu G., Shi N., Tang D., Ferdek P.E., Jakubowska M.A., Liu S., Zhu X., Zhang J., Yao L., Sang X., Zou S., Liu T., Mukherjee R., Criddle D.N., Zheng X., Xia Q., Berggren P.O., Huang W., Sutton R., Tian Y., Huang W, & Fu X. (2022). A microRNA checkpoint for Ca2+ signaling and overload in acute pancreatitis. Molecular Therapy, 30(4), 1754-1774.

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PDMS Microfluidic Flow Focusing Synthesis

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Polydimethylsiloxane (PDMS) microfluidic devices were fabricated by well-established soft lithography approaches [67 (link)]. A flow focusing device was fabricated comprising a cross-flow junction followed by a longer channel with dimensions 500 μm width and 120 μm height. Inlet and outlets consisted of fluorinated ethylene propylene tubing (Cole Parmer, St Neots, UK, FEP) directly inserted into the PDMS and further sealed with rapid epoxy adhesive. Aqueous NaCMC solutions were injected as the dispersed phase and oleic acid as the carrier phase with a syringe pump (Harvard Apparatus, Cambourne, UK, Pump 33 DDS) at total flow rates between 0.5 and 100 μL min⁻¹, where the dispersed phase flow rate was maintained at a lower rate than the carrier phase to produce various droplet sizes. Outlet tubing was either immersed into a reservoir of FeCl₃ solution at a prescribed concentration or connected to a T-junction (Kinesis, St Neots, UK, 0.57 μL swept volume) with FeCl₃ solution injected orthogonal to the flow direction. Optical microscopy was performed on an inverted transmission microscope (Olympus, UK, IX71) with 4X and 10X infinity-corrected objectives attached to a CMOS camera (Basler AG, Ahrensburg, Germany, acA1300-200uC).

Sharratt W.N., Lopez C.G., Sarkis M., Tyagi G., O’Connell R., Rogers S.E, & Cabral J.T. (2021). Ionotropic Gelation Fronts in Sodium Carboxymethyl Cellulose for Hydrogel Particle Formation. Gels, 7(2), 44.

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Sodium Taurocholate-Induced Acute Pancreatitis

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Mice were divided into control, AP and AP+SMI group, respectively (n = 8 in each group). AP-model mice were induced via retrograde pancreatic ductal injection with 4% sodium taurocholate (NaT) dissolved in saline. Briefly, mice were anesthetized with 2% isoflurane (gas flow: 0.5 L/min) using a vaporizer and gas scavenger system. Using sterile techniques, a small midline laparotomy incision was made. The duodenum was flipped to reveal its distal side and held in place by ligatures. The bile duct was identified, and a 30-gauge needle was inserted through the antimesenteric aspect of the duodenum to cannulate the biliopancreatic duct. NaT was infused at 5 μL/min for 10 min using a perfusion pump (Pump33DDS, Harvard Apparatus, Holliston, MA, USA). Saline-infused mice served as controls. In the AP+SMI group, SMI (10 mL/kg) was injected intraperitoneally twice: pretreated within 24 h before NaT induction and 1 h after the procedure. Animals were euthanized 24 h after NaT induction, and relevant organs were harvested to assess the severity. Detailed protocols for the histopathological assessment of the pancreas were as previously described.

He Y., Hu C., Liu S., Xu M., Liang G., Du D., Liu T., Cai F., Chen Z., Tan Q., Deng L, & Xia Q. (2022). Anti-Inflammatory Effects and Molecular Mechanisms of Shenmai Injection in Treating Acute Pancreatitis: Network Pharmacology Analysis and Experimental Verification. Drug Design, Development and Therapy, 16, 2479-2495.

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