All culture media including M199, α-MEM, M5, Trypsin (0.25%)-EDTA (0.02%) and PBS, as well as Penicillin (10,000 U/ml)-Streptomycin (10,000 μg/ml) concentrate solution were prepared and supplied by the Memorial Sloan-Kettering Cancer Centre Culture Media Core Facility (New York, NY). Amphoteracin B was purchased from Life Technologies (Grand Island, NY). Gelatin was from TJ Baker Inc (Philipsburgh, NJ). Bovine serum albumin was from Gemini Bioproducts (West Sacramento, CA). Falcon tissue culture flasks and centrifuge tubes were purchased from BDBiosciences (Two Oak Park, Bedford, MA). HDF were from The Coriell Institute (Camden, NJ). SAOS-2 osteosarcoma cells were from the American Type Culture Collection (VA, USA). A673 osteosarcoma and H3122 lung carcinoma cells were from the collection at the Memorial Sloan Kettering Cancer Center. The lipophilic fluorescent probes DiD (excitation 644nm, emission 665nm) and DiO (excitation 484nm, emission 501nm) Vybrant cell labelling solutions were from Molecular Probes, Life Technologies (Grand Island, NY). DAPI was provided by the FACS core facility at Memorial Sloan Kettering Cancer Center.
Saos 2 osteosarcoma cells
Manufactured by American Type Culture Collection
Sourced in United States
SAOS-2 osteosarcoma cells are a human cell line derived from a primary osteosarcoma. They are adherent cells that can be cultured in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Centrifuge tube, by BD (2 mentions)
Falcon tissue culture flasks, by BD (2 mentions)
Amphoteracin b, by Thermo Fisher Scientific (2 mentions)
Vybrant cell labelling solutions, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Gemini Bio (1 mentions)
Dulbecco s modified eagle medium dmem, by Euroclone (1 mentions)
Hct116 colorectal carcinoma cells, by American Type Culture Collection (1 mentions)
Hela cervical adenocarcinoma cells, by American Type Culture Collection (1 mentions)
Saos2 cell line, by American Type Culture Collection (1 mentions)
Anti mouse igg hrp, by BD (1 mentions)
Mt1 mmp, by Santa Cruz Biotechnology (1 mentions)
Transwell invasion chambers, by Promega (1 mentions)
Anti rabbit igg hrp, by BD (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
5 protocols using saos 2 osteosarcoma cells
1
Fluorescent Labeling of Cell Lines
2
Synthesis and Evaluation of Gold Complexes
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KAuBr4⋅2H2O and KAuCl4⋅2H2O were purchased from Alfa Aesar, [D6]DMSO from Aldrich, ammonium pyrrolidinedithiocarbamate from Fluka, and P4O10 from AnalaR Normapur–Prolabo. Dulbeccos modified Eagle medium (DMEM) was purchased from Euroclone. All other reagents and solvents (from Aldrich or Fluka) were of high purity and used without further purification. SAOS-2 osteosarcoma cells, HeLa cervical adenocarcinoma cells, and HCT116 colorectal carcinoma cells were purchased from the American Type Culture Collection (ATCC). MEF-NF1−/− cells were kindly provided by Prof. Reuven Stein (George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel).
3
Culturing Human Dermal Fibroblasts and Osteosarcoma Cells
4
Fibroblast and Cancer Cell Sources
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Human dermal fibroblasts from The Coriell Institute (Camden, NJ, USA) were used in experiments performed at MSKCC, and from the American Type Culture Collection (Manassas, VA, USA) in experiments conducted at the CMPRU. The only exception was for experiment ‘c’ in the CMPRU, where human gingival fibroblasts were used and obtained from explant cultures as previously described [1 (link)]. SAOS-2 osteosarcoma cells were from the American Type Culture Collection (Manassas, VA, USA), and a SAOS-2 cell line expressing GFP was as earlier reported and used in experiment ‘i’ [1 (link)]. Further cancer cell lines studied were from the collection at the Westmead Institute of Medical Research (Westmead, NSW, Australia) and shared between collaborating laboratories of the CMPRU and MSKCC.
5
Osteosarcoma Cell Invasion and Wnt Signaling
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MG-63 and Saos-2 osteosarcoma cells were purchased from the American Type Culture Collection (Manassas, VA, USA) cultured in RPMI-1640 (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin, in a 5% CO2 humidified atmosphere at 37°C. α1-PDX (126850-2.5MG; Calbiochem-Merck KGaA, Darmstadt, Germany) was added to the medium at a concentration of 480 nM where indicated. Primary antibodies against Wnt, β-catenin, MT1-MMP and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Other reagents were used, including anti-mouse-IgG-HRP and anti-rabbit-IgG-HRP (BD Biosciences, CA, USA) and Transwell invasion chambers (Promega, Madison, WI, USA).
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