The fentanyl and ketamine hydrochloride injections were purchased from Yichang Humanwell Pharmaceutical Co., Ltd. (Yichang, China). The malondialdehyde enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D Systems, Inc. (Minneapolis, MN, USA). The tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-10 ELISA kits were purchased from Jiancheng Bioengineering Research Institute (Nanjing, China). The cardiac troponin (cTnI), creatine kinase and myoglobin (Mb) ELISA kits were purchased from Beyotime Institute of Biotechnology (Haimen, China). The terminal deoxynucleotidyl-transferase dUTP nick end-labeling (TUNEL) kit was purchased from Roche Diagnostics GmbH (Mannheim, Germany). Atropine, propofol, heparin and lysis buffer were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium dodecyl sulfate buffer, polyacrylamide gels, nitrocellulose membranes, Tris-buffered saline containing Tween-20 (TBST), enhanced chemiluminescence (ECL) solution, 4% paraformaldehyde, paraffin, Triton X-100, phosphate-buffered saline (PBS) and the Bicinchoninic Acid Protein Assay kit were purchased from Kangchen Bio-tech, Inc. (Shanghai, China). Potassium chloride (KCl) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China)
Terminal deoxynucleotidyl transferase dutp nick end labeling tunel kit
Manufactured by Roche
Sourced in Switzerland, Germany, United States
The Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit is a laboratory tool used to detect and quantify DNA fragmentation. The kit utilizes an enzymatic reaction to label the free 3'-hydroxyl termini of fragmented DNA, allowing for the identification of cells undergoing apoptosis or cell death.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (2 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Thapsigargin, by Merck Group (2 mentions)
Caspase 3, by Cell Signaling Technology (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Heparin, by Merck Group (1 mentions)
Lysis buffer, by Merck Group (1 mentions)
Propofol, by Merck Group (1 mentions)
Potassium chloride (kcl), by Sinopharm (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Polyclonal cleaved caspase 3, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Foxo1 antibody, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase labeled secondary antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Annexin 5 phycoerythrin pe apoptosis detection kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (1 mentions)
Anti β actin antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
16 protocols using terminal deoxynucleotidyl transferase dutp nick end labeling tunel kit
1
Fentanyl and Ketamine Injection Impacts on Cardiac Biomarkers
2
Liriodenine Cytotoxicity Evaluation
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Liriodenine was purchased from ChemBest Research Laboratories, Ltd. (Shanghai, China). All the general reagents used for the cell culture were purchased from Gibco (Carlsbad, CA, USA). Hoechst 33342 and the lectin dyes were purchased from Sigma-Aldrich (St. Louis, MO, USA). The rabbit anti-human monoclonal p53 (1:500 dilution), polyclonal -vascular epidermal growth factor (VEGF; 1:1,000 dilution), polyclonal -cleaved caspase-3 (1:500 dilution) and monoclonal -β-actin (1:5,000 dilution) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). The rabbit anti-human monoclonal B-cell lymphoma 2 (Bcl-2; 1:500 dilution) and polyclonal -Bcl-2-associated X protein (BAX; 1:500 dilution) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Peroxidase-conjugated goat anti-rabbit immunoglobulin G was purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Roche (Basel, Switzerland).
3
Lentiviral Modulation of AQP9 in Hepatoma Cells
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Human hepatoma cell line SMMC7721 was purchased from the Cell Bank at the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. Lentivirus vector expressing AQP9-enhanced green fluorescent protein (eGFP) (named LV-AQP9) and lentivirus vector expressing eGFP (named LV-PWPI) were obtained from Shanghai Genechem Co., Ltd. (Shanghai, China). Annexin V-phycoerythrin (PE) apoptosis detection kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Roche (Basel, Switzerland). Cell counting kit-8 (CCK8) assay was purchased from Dojindo (Tokyo, Japan). Rabbit anti-human AQP9 antibody was purchased from Abcam (Cambridge, MA, USA). Rabbit anti-human caspase-3, PI3K, proliferating cell nuclear antigen (PCNA), Akt, P-Akt, and forkhead box protein O1 (FOXO1) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody and horseradish peroxidase-labeled secondary antibodies were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China).
4
Characterization of GEM-loaded Nanoparticles
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GEM (crude drug) was obtained from Eli Lilly and Company (Indianapolis, IN, USA). GEM-C14 and GEM-HSA-NP were prepared as reported previously.21 (link) NBMPR was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Normal saline (NS) was purchased from Shanghai Baxter Healthcare Co., Ltd. (Shanghai, China). Roswell Park Memorial Institute (RPMI)-1640 culture medium, fetal bovine serum and Dulbecco’s phosphate-buffered saline (DPBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Cell-Counting Kit-8 (CCK-8) was purchased from Dojindo (Kyushu, Japan). Annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7-AAD) kit and propidium iodide (PI) kit were purchased from BD Biosciences (San Jose, CA, USA). RNeasy Mini kit was purchased from Qiagen NV (Venlo, the Netherlands). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Hoffman-La Roche Ltd. (Basel, Switzerland).
5
Cardiac Tissue Histology and Staining
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Hearts were dehydrated in 30% sucrose solution, embedded in Tissue-Tek® Optimal Cutting Temperature Compound (Sakura Finetek, Torrance, CA, USA), snap-frozen in liquid nitrogen, and cut into sections of thickness 5 μm for staining. Sections were washed with phosphate-buffered saline, permeabilized with 0.2% Triton X (Sigma–Aldrich) and blocked with 5% bovine serum albumin (Gibco, Billings, MT, USA). Apoptosis was evaluated with a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit (Roche Applied Science, Basel, Switzerland); cell proliferation was incubated with ki67 (Abcam, United Kingdom) and ROSs was stained with dihydroethidium (DHE, Beyotime Biotechnology, China). Cardiomyocytes were stained with troponin I (Abcam, United Kingdom) and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA, USA).
6
Evaluation of Apoptosis-Related Biomarkers
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KTC (lot number 130705) is a product of Guiyang Xintian Pharmaceutical Limited Company (Guiyang, Guizhou province, China). The estradiol (E2), progesterone (P), interleukin-6 (IL-6), cancer antigen (CA125), and cyclooxygenase-2 (COX-2) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu province, China). The tumor necrosis factor-alpha (TNF-α) ELISA kit was purchased from R&D Systems, Inc. (Minneapolis, USA). BCA Protein Assay Kit (P0012S) was bought from Beyotime Biotechnology (Shanghai, China). Rabbit polyclonal caspase-8 (ab25901), caspase-9 (ab2324), cytochrome c (ab90529), and active and procaspase-3 (ab13847) were bought from Abcam Biotechnology (Cambridge, USA). The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kit (11684795910) was purchased from Roche (Basel, Switzerland). An enhanced chemiluminescence (ECL) detection kit was purchased from Boster (Wuhan, Hubei province, China).
7
Gemcitabine Cytotoxicity Evaluation
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Gemcitabine (crude drug) was obtained from Eli Lilly and Company (Indianapolis, IN, USA), and the purity was >99.9%. HSA (20%, 50 mL) was purchased from Baxter AG (Vienna, Austria). Normal saline (NS) was purchased from Shanghai Baxter Healthcare Co., Ltd. (Shanghai, People’s Republic of China). Absolute ethanol and chloroform were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China); deionized water was purchased from Fudan University (Shanghai, People’s Republic of China); and Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Double stain Hoechst/PI kit and myristic acid (C14) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kyushu, Japan). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Hoffman-La Roche Ltd. (Basel, Switzerland). Phosphate-buffered saline (PBS) and other reagents were prepared in our laboratory. All the solvents and chemicals were of analytical grade.
8
TUNEL Assay for Apoptosis Quantification
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The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Roche ( Indiana, USA) and the assay was performed according to the manufacture's instruction for both non-adherent and adherent cells. Briefly, unstimulated, TNFα stimulated and TNFα stimulated and FasL treated RPTEC cells were washed before the adherent cells were fixed in 4% paraformaldehyde (PFA) in PBS for 10 min on ice. The non-adherent cells were fixed with 2% PFA for 60 min in room temperature prior to centrifugation and removal of supernatant. The cells were permeabilized using 0.1% Triton X-100 (VWR International) in PBS for 5 min. The cells were subsequently treated with TUNEL Mix containing TUNEL label (11767291910; Roche) and the terminal deoxynucleotidyl transferase enzyme (11767305001; Roche), and incubated at 37°C for 1 h. The cells were washed with PBS and nuclei were stained by DAPI and subsequently subjected to confocal microscopy using the Carl Zeiss LSM 700 confocal microscopy. As a positive control in the TUNEL assay, RPTEC were exposed to 10U recombinant DNase I to induce single-stranded (TUNEL-positive) nicks, and the negative control was represented by TUNEL in absence of terminal deoxynucleotidyl transferase.
9
Cytotoxicity Evaluation of Compounds
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N-Octyl isocyanate and hydrogen peroxide (H2O2), dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Aladdin (Shanghai, China). Cisplatin (purity 99%) was bought from Kunming Institute of Precious Metals (Yunnan, China). Human serum albumin (HSA) was purchased from Shang Hai Yuan Ye Bio-Technology Co., Ltd (Shanghai, China). 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI), Alexa Fluor 488 (Alex 488), Cy5.5, and Cy7.5 were purchased from Sigma-Aldrich (Shanghai, China). Calcein AM/PI (acetyl methoxy methyl ester/propidium iodide) cell viability kit, DNA extraction kit and Annexin V-FITC apoptosis detection kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit were purchased from Roche (Basel, Switzerland). RPMI-1640 medium, DMEM, 0.25% trypsin–EDTA, fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were purchased from Gibco (NY, USA). Cell culture vessels were purchased from Corning (NY, USA).
10
Glioma Cell Line Transfection Optimization
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The human glioma cell lines U251 and the GFP-Cx43 plasmid were obtained from our laboratory inventory. IMDM culture medium was purchased from Gibco (Grand Island, NY, USA). Streptomycin, Lipofectamine 2000, and TRIzol were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the ECL Light-emitting Substrate Kit was purchased from Thermo (Waltham, MA, USA). The Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Kit was purchased from Roche (Basel, Switzerland).
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