Phenazinemethanesulphonate

Dihydroquercetin (Disto-Pharmaceuticals, India), LPS (E. coli, L3129), bovine serum albumin, and griess reagent were procured from Sigma-Aldrich (St. Louis, MO, USA). thiobarbituric acid (TBA), NADH, sodium succinate, sodium azide, phenazinemethanesulphonate (PMS) and nitro blue tetrazolium (NBT) were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents of high-performance liquid chromatography (HPLC) and analytical grade were procured from local suppliers.

Streptozotocin, tetra methyl rhodamine methyl ester (TMRM), glutamate, and o-phthalaldehyde (OPA) were procured from Sigma-Aldrich (St. Louis, MO, USA). Glibenclamide (standard drug) was obtained as a gift sample from Accent pharma (QC. Ref. No. GLB/B129/10/11) Pvt. Ltd. Thiobarbituric acid (TBA), ethylene glycol tetra-acetic acid (EGTA), and 2-[4-(2-hydroxyethyl)1-piperazinyl]ethanesulfonic acid (HEPES buffer, acid free) were purchased from Hi-media (Mumbai) and sodium succinate, sodium azide, phenazine methanesulphonate (PMS), and nitro blue tetrazolium were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents were procured from local suppliers and were of analytical grade. Plasma insulin was assayed by using commercial enzyme-linked immunosorbent assay kit (ELISA, Boerhringer Mannheim, Germany).
Total cholesterol (TC), high density lipoprotein (HDL) cholesterol, triglyceride (TG), blood urea nitrogen (BUN), creatinine (CRTN), and total protein (TPR) were estimated using kits from Span Diagnostics Ltd., India.