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Apc conjugated anti mouse cd11c

Manufactured by Thermo Fisher Scientific
Sourced in United States

APC conjugated anti-mouse CD11c is a fluorochrome-labeled antibody that binds to the CD11c antigen, a cell surface marker expressed on mouse dendritic cells. This product can be used in flow cytometry applications to identify and enumerate dendritic cell populations in mouse samples.

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3 protocols using apc conjugated anti mouse cd11c

1

Quantifying SIINFEKL-Presenting DCs in dLN

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To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
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2

Multicolor Flow Cytometry Panel

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APC-conjugated anti-mouse CD11c; PE-conjugated anti-mouse MHC-II and CD11b; PE-Cy5-conjugated anti-mouse CD86; FITC-conjugated anti-mouse CD40, CD80, CD83, and GR-1, and PE-conjugated anti-mouse IFN-γ monoclonal antibodies were purchased from eBioscience (San Diego, CA, USA). Polyclonal anti-HO-1 Ab was purchased from Enzo Life Sciences (Lausen, Switzerland). Polyclonal anti-tubulin Ab was purchased from Beyotime (Shanghai, China).
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3

Quantifying SIINFEKL-Presenting DCs in dLN

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
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