Radix Tetrastigma Hemsleyani Flavone (RTHF) was synthesized in the Key Laboratory Research and Development of New Drugs of Traditional Chinese Medicine of Zhejiang Province. Our experiments used the following: FBS and RPMI 1640 (HyClone), MTT (Beyotime), Annexin V-FITC Apoptosis Detection Kit (Keygen), Transwell chamber (Corning, USA), Matrigel (BD Biosciences USA), Proteasome-Glo Chymotrypsin-like Cell-Based Assay kit, DUB-Glo Protease Assay kit (Promega, USA), human 19S proteasome (Boston Biochem), and the antibodies to Bax, Bcl-2, Caspase-3/9, ub-prs, USP14, UCHL5, and POH1 (CST).
Human 19s proteasome
Manufactured by R&D Systems
Sourced in United States
The Human 19S proteasome is a key regulatory component of the proteasome complex, which is responsible for the degradation of ubiquitinated proteins in eukaryotic cells. The 19S proteasome acts as a gatekeeper, recognizing, unfolding, and translocating targeted proteins into the 20S proteasome core for degradation.
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Lab products found in correlation
2 protocols using human 19s proteasome
1
RTHF Inhibits Cancer Cell Proliferation
2
Isopeptide Cleavage by Human 19S Proteasome
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Example 32
200 μM isopeptide-containing compound will be incubated with 1 μg human 19S proteasome (Boston Biochem, E-366) in 200 μM Hepes pH 7.5, 200 μM NaCl, 10 μM dithiolthreitol and 0.2% glycerol in a 50 μl reaction volume at 37° C. for 5, 10, 20 and 40 minutes. Samples will be snap frozen in on dry ice. The samples will be thawed, and trifluoroacidic acid (TFA) will be added. The samples will be analyzed by LC-MS to establish cleavage of the isopeptide moiety to provide ATRN 6-A and/or ATRN 6-B or ATRN 7-A and/or ATRN 7-B.
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