The 4 radioactive water springs Agricola (water resource yield 10 l/min., temperature 29°C, radioactivity 20 kBq/l), Běhounek (300 l/min., 36°C, 10 kBq/l), C1 (30 l/min., 29°C, 11 kBq/l), and Curie (30 l/min., 29°C, 5 kBq/l) spring up at a depth of 500 m below the surface of gneiss mountain bedrock, in the former silver-uranium mine Svornost (Jáchymov, Czech Republic; GPS 50°22′22.9″N 12°54′42.1″E). The chemical composition of the water springs is summarized in Table S1. Under sterile conditions, 20 liters of each spring water was collected and separately filtered with low vacuum through Merck Millipore Sterito Sterile Vacuum Bottle-Top Filters with PES filter membranes with a 0.2 μm pore size (Fisher Scientific). Filtered water (filtrate) was preserved for further experiments overnight at 28°C or 37°C (according to the spring’s temperature). Filter membranes with retained cells were excised from the plastic cup using a sterile pair of tweezers, placed into 25 mL of sterile filtrate of the respective spring water, and incubated for 48 h at 120 rpm.
Merck millipore sterito sterile vacuum bottle top filters with pes filter membranes
Manufactured by Thermo Fisher Scientific
The Merck Millipore Sterito Sterile Vacuum Bottle-Top Filters with PES filter membranes are designed for the sterile filtration of solutions. They feature a PES (polyethersulfone) filter membrane that retains microorganisms while allowing the passage of fluids. The filters are pre-sterilized and ready for use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using merck millipore sterito sterile vacuum bottle top filters with pes filter membranes
1
Isolation and Characterization of Jáchymov Springs
2
Isolation and Characterization of Jáchymov Springs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 4 radioactive water springs Agricola (water resource yield 10 l/min., temperature 29°C, radioactivity 20 kBq/l), Běhounek (300 l/min., 36°C, 10 kBq/l), C1 (30 l/min., 29°C, 11 kBq/l), and Curie (30 l/min., 29°C, 5 kBq/l) spring up at a depth of 500 m below the surface of gneiss mountain bedrock, in the former silver-uranium mine Svornost (Jáchymov, Czech Republic; GPS 50°22′22.9″N 12°54′42.1″E). The chemical composition of the water springs is summarized in Table S1. Under sterile conditions, 20 liters of each spring water was collected and separately filtered with low vacuum through Merck Millipore Sterito Sterile Vacuum Bottle-Top Filters with PES filter membranes with a 0.2 μm pore size (Fisher Scientific). Filtered water (filtrate) was preserved for further experiments overnight at 28°C or 37°C (according to the spring’s temperature). Filter membranes with retained cells were excised from the plastic cup using a sterile pair of tweezers, placed into 25 mL of sterile filtrate of the respective spring water, and incubated for 48 h at 120 rpm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!