Pe labeled anti mouse cd3

Single-cell suspensions were stained with fluorochrome-labeled monoclonal antibodies after incubation with FcR-blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany). The following antibodies (all purchased from BioLegend) were used for flow cytometry: FITC-labeled anti-mouse CD11b, PE-labeled anti-mouse F4/80, PE/Cy7-labeled anti-mouse CD206, APC-labeled anti-mouse CD80, APC/Cy7-labeled anti-mouse CD45, FITC-labeled anti-mouse B220, PE-labeled anti-mouse CD3, PE/Cy7-labeled anti-mouse CD4, APC-labeled anti-mouse CD8, PE/Cy7-labeled anti-mouse CD138, APC-labeled anti-mouse CD19. Flow cytometric data were acquired on FACSVerse (BD Biosciences, San Jose, CA, USA) and then were analyzed using FlowJo software (TreeStar).

Todd Hewitt broth, yeast extract, trypticase soy broth (TSB) and Bacto^TM agar were purchased from BD Biosciences (Franklin Lakes, NJ, USA). Blood agar plates were purchased from Hanil Komed (Seongnam, Korea). 2,2,2-Tribromoethyl alcohol, 2-methyl-2-butanol, Red Blood Cell Lysis Buffer, and hematoxylin were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Goat anti-mouse IgG Fc-, IgG1-, IgG2b-, and IgA-HRP were purchased from Southern-Biotech (Birmingham, AL, USA). A Mouse Dendritic Cell Enrichment Set was purchased from BD Biosciences (San Diego, CA, USA). The following antibodies were purchased from BioLegend (San Diego, CA, USA): APC-labeled anti-mouse IFN-γ, APC-labeled anti-mouse IL-4, PE-labeled anti-mouse IL-17A, PE-labeled anti-mouse CD3, APC-labeled anti-mouse CD8, PerCP-labeled anti-mouse CD11c, FITC-labeled anti-mouse I-A^b for MHC class II, APC-labeled anti-mouse CXCR5, PerCP-labeled anti-mouse B220, PE-labeled anti-mouse CD69, PE-labeled anti-mouse PD-1, PE-labeled anti-mouse CD80, and FITC-labeled anti-mouse CD86 antibodies. FITC-labeled anti-mouse CD4 antibody was obtained from BD Biosciences. All of the isotypes matched with each antibody were purchased from BioLegend or BD Biosciences.