Spss statistics 17.0 software package

Manufactured by IBM

SPSS Statistics 17.0 is a software package designed for statistical analysis. It provides a comprehensive set of tools for data management, analysis, and reporting. The software is capable of handling a wide range of data types and offers a variety of statistical techniques, including descriptive statistics, regression analysis, and hypothesis testing.

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2 protocols using spss statistics 17.0 software package

Comprehensive Bacterial Diversity Analysis

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Sequences were selected to estimate the total bacterial diversity of the DNA samples in a comparable manner and were trimmed to remove barcodes, primers, chimeras, plasmids, mitochondrial DNA, and any non-16S bacterial reads and sequences < 150 bp. The MG-RAST (Meyer et al., 2008 (link)) with the RDP was used for analyses of all sequences. The pipeline takes in bar-coded sequence reads, separates them into individual communities by bar code, and utilizes a suite of external programs to make taxonomic assignments RDP database (Wang et al., 2007 (link)) and estimates phylogenetic diversity, with a minimum e-value of 1e-5, a minimum identity of 60%, and a minimum alignment length of 15 measured in base pairs for RNA databases. Each value obtained indicated the percentage of relative frequency of reads with predicted proteins and ribosomal RNA genes annotated to the indicated taxonomic level. The output file was further analyzed using SPSS Statistics 17.0 Software Package (SPSS Inc.) and Statistical Analysis of Metagenomic Profiles (STAMP) software package version 2.1.3 (Parks et al., 2014 (link)).

Rodríguez-Nogales A., Algieri F., Garrido-Mesa J., Vezza T., Utrilla M.P., Chueca N., Fernández-Caballero J.A., García F., Rodríguez-Cabezas M.E, & Gálvez J. (2018). The Administration of Escherichia coli Nissle 1917 Ameliorates Development of DSS-Induced Colitis in Mice. Frontiers in Pharmacology, 9, 468.

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Statistical Analysis of Paired Samples

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Statistical analysis was carried out using the SPSS Statistics 17.0 Software Package (SPSS Inc.). Wilcoxon matched-pairs signed rank test (two-tailed) was performed to analyze changes between various time points in the same cohort, while Independent Mann–Whitney U test (two-tailed) was performed to analyze the differences between groups

Zhang C., Yin A., Li H., Wang R., Wu G., Shen J., Zhang M., Wang L., Hou Y., Ouyang H., Zhang Y., Zheng Y., Wang J., Lv X., Wang Y., Zhang F., Zeng B., Li W., Yan F., Zhao Y., Pang X., Zhang X., Fu H., Chen F., Zhao N., Hamaker B.R., Bridgewater L.C., Weinkove D., Clement K., Dore J., Holmes E., Xiao H., Zhao G., Yang S., Bork P., Nicholson J.K., Wei H., Tang H., Zhang X, & Zhao L. (2015). Dietary Modulation of Gut Microbiota Contributes to Alleviation of Both Genetic and Simple Obesity in Children. EBioMedicine, 2(8), 968-984.

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