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Nitric oxide

Manufactured by Nanjing Jiancheng
Sourced in China

Nitric oxide is a colorless gas that serves as a key signaling molecule in the human body. It plays a crucial role in regulating various physiological processes, such as vasodilation, neurotransmission, and immune response. This lab equipment product can be used to measure and study the production and function of nitric oxide in research and clinical settings.

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4 protocols using nitric oxide

1

Cell Signaling Pathway Modulation

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Nitro blue tetrazolium chloride (N-BT) was purchased from the medicine supply station of the Academy of Military Medical Sciences (China). Lactate dehydrogenase (LDH), malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase (NOS), IL-1β, PI3K, and nuclear factor kB (NF-kB) kits were purchased from Nanjing Jiancheng Bioengineering Institute (China). High glucose Dulbecco's modified Eagle's medium (DMEM-H) and fetal bovine serum (FBS) were purchased from Gibco. MicroRotofor™ cell lysis kit and Bio-Plex Pro™ cell signaling MAPK panel were purchased from Bio-Rad (USA). Proteasome inhibitor, MG132, and IKK (inhibitor of nuclear factor kappa-B kinase, IκB) inhibitor, parthenolide, were purchased from Sigma.
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2

Amomum tsao-ko Hepatoprotective Effects

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Amomum tsao-ko (Hebei yishengshan traditional Chinese Medicine Co., Ltd.); GM sulfate injection; verapamil (abbvie Deutschland GmbH & Co. KG, Düsseldorf, Germany); creatinine (Cr), urea nitrogen (BUN), a urine protein test kit, lysozyme (LZM), β-b-acetyl-glucosaminidase (NAG), nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (SOD), malondialdehyde (MDA), and total protein quantitative test kit (BCA) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); rat IL-6, rat IL-1β, and a rat TNF-α ELISA kit were purchased from Lianke Biology Co. Ltd., Hangzhou, China; P-SAPK/JNK antibody, p38/P-p38, NF-κB p65 were purchased from Cell Signaling Technology; ERK1/2 antibody, P-ERK1 + ERK2, caspase-3, Bax, Bcl-2, Goat anti-rabbit antibody, and Goat anti-mouse antibody were purchased from Wuhan bode Biology Co., Ltd., Wuhan, China; and GADPH TA-08 and β-Actin TA-09 were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., Shanghai, China.
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3

Afrocyclamin A's Anti-Inflammatory Mechanisms

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Afrocyclamin A was received as a gift sample. Bafilomycin A1, 3-Methyladenine (3-MA),
ammonium chloride and chloroquine were purchased from the Sigma Aldrich, USA. Transforming
growth factor beta 1 (TGF-β1) as purchased from Peprotech Inc. (Rocky Hill, NJ, USA).
Pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β),
IL-6 were purchased from the eBioscience (San Diego, CA, USA). Counting Kit-8 (CCK-8) (CK04)
was purchased from Dojindo Molecular Technologies, Inc., USA; superoxide dismutase (SOD),
glutathione (GSH), malonaldehyde (MDA), catalase (CAT) and nitric oxide (NO) were purchased
from Jiancheng Bioengineering Institute (Nanjing, China). Collagen, type, α-actin,
microtubule-associated protein 1light chain 3 (LC3), β-catenin and histone antibodies were
purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for Beclin-1, Atg5 and
osteocalcin were purchased from Epitomics (Burlingame, CA, USA).
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4

Baicalin and Baicalein in Enterotoxigenic E. coli Treatment

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Baicalin (CAS number 21967-41-9), baicalein (CAS number 491-67-8), and sodium hydroxymethylcellulose (CMC-Na; CAS number 9085-26-1) were purchased from Sinopharm Co., Ltd. Pentabarbital (CAS number 57-33-0), alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), triglyceride (TG), cyclic guanosine monophosphate (cGMP), cyclic adenosine monophosphate (cAMP), sodium-potassium adenosine triphosphatase (Na+K+-ATPase), interleukin-1β (IL-1β), interleukin-17 (IL-17), and interleukin-6 (IL-6) kits were provided by Nanjing Jiancheng Bioengineering Institute. Sulfasalazine, Red Star Erguotou liquor, and honey were purchased from Jingdong e-market. Enterotoxigenic Escherichia coli (E. coli; No.: ATCC 25922) was provided by the National Institutes for Food and Drug Control of China. The nutrient agar was obtained from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, China). All reagents used were analytical grade.
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