Xterra ms c18 column

Manufactured by Waters Corporation

Sourced in United States, France

The XTerra MS C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a bonded C18 stationary phase that provides efficient and reproducible separations. The column is suitable for a variety of applications, including the analysis of small molecules, peptides, and pharmaceutical compounds.

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Lab products found in correlation

74 protocols using xterra ms c18 column

Optimized LC-MS Analysis of Compounds

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Waters Alliance 2695 HPLC system was used in the LC-MS analysis using an XTerra MS C18 column (2.1 × 100 mm, 3.5 µm particle size; Waters, MA, USA). The injection volume was 30 µL. The mobile phase A was methanol, while the mobile phase B was water with the following linear gradient programme: the concentration of mobile phase A was 10% at 0 min, 25% at 5 min, increased to 75% with gradient 5%/min and held for 5 min, then decreased to 10% and held for 15 min, while the mobile phase B was 90% at 0 min, 75% at 5 min, decreased to 25% and held for 5 min with gradient 5%/min, then increased until 90% and held for 15 min. The flow rate was 0.2 mL/min and the column temperature was 40 °C. The spectra were monitored using Waters Quattro Micro™ in full scan mode (m/z 50–1200) and electrospray ionization (ESI) interface in positive mode, with the source temperature maintained at 120 °C, desolvation temperature at 450 °C, and gas flow of 500 L/h. This procedure was controlled by Masslynx software (Version 4.0).

Hanifah A., Maharijaya A., Putri S.P., Laviña W.A, & S.o.b.i.r. (2018). Untargeted Metabolomics Analysis of Eggplant (Solanum melongena L.) Fruit and Its Correlation to Fruit Morphologies. Metabolites, 8(3), 49.

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High pH Reversed-Phase Fractionation of TMT-Labeled Peptides

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The hpRP chromatography was carried out using a Dionex UltiMate 3000 HPLC system with the built-in micro fraction collection option in its autosampler and UV detection (Sunnyvale, CA). The TMT 6-plex tagged tryptic peptides were reconstituted in buffer A (20 mM ammonium formate pH 9.5), and loaded onto an XTerra MS C₁₈ column (3.5 µm, 2.1 × 150 mm, Waters, Milford, MA, USA) with 20 mM ammonium formate (NH₄FA), pH 9.5 as buffer A and 80% ACN/20% 20 mM NH₄FA as buffer B. Forty-eight fractions were collected at one-minute intervals and pooled into a total of 12 fractions based on the UV absorbance at 214 nm and with multiple fraction concatenation strategy. All of the fractions were dried and reconstituted in 2% ACN/0.5% FA for nano-liquid chromatography (LC)-MS/MS analysis in an LTQ-Orbitrap Velos (Thermo-Fisher Scientific, San Jose, CA, USA) mass spectrometer equipped with a “CorConneX” nano-ion source (CorSolutions LLC, NY, USA) as reported previously [93 (link)].

Liu Y., Ji D., Turgeon R., Chen J., Lin T., Huang J., Luo J., Zhu Y., Zhang C, & Lv Z. (2019). Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress. International Journal of Molecular Sciences, 20(10), 2486.

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HPLC Separation of Muropeptides

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Muropeptide separation by HPLC was performed with an Xterra MS C18 column (Waters) and UV detection at 210 nm. Elution relied on a gradient from 100% buffer A (sodium phosphate buffer 50 mM pH 4.3) to 100% buffer B (sodium phosphate buffer 50 mM pH 4.9 and methanol 15% (v/v)) over 100 min.

Lin C.S., Chan A.C., Vermeulen J., Brockerman J., Soni A.S., Tanner M.E., Gaynor E.C., McIntosh L.P., Simorre J.P, & Murphy M.E. (2021). Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs helical cell shape of Campylobacter jejuni. The Journal of Biological Chemistry, 296, 100528.

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Identification of Steroidal Alkaloids in Veratrum californicum

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To identify the steroidal alkaloids in V. californicum aerial and root/rhizome extracts, samples were analyzed by HPLC-MS, where the mass spectrometer was an ultra-high resolution Quadrupole Time of Flight (QTOF) instrument (Bruker maXis, Billerica, MA, USA). The electrospray ionization (ESI) source was operated under the following conditions: positive ion mode, 1.2 bar nebulizer pressure, 8 L/min flow of N₂ drying gas heated to a temperature of 200°C, 3000 V to −500 V voltage between HV capillary and HV end-plate offset, mass range set from 80 to 800 m/z, and the quadrupole ion energy at 4.0 eV. Sodium formate was used to calibrate the system in this mass range. HPLC separation was achieved using a XTerra MS C₁₈ column, 3.5 μm, 2.1 × 150 mm (Waters, Milford, MA, USA). The flow rate was 250 μL/min. The mobile phases were 5% acetonitrile and 0.1% formic acid in water (Buffer A) and acetonitrile and 0.1% formic acid (Buffer B). The linear gradient method was used to separate analytes starting at 5% Buffer B and increasing to 60% Buffer B over 25 min. A 1 μL sample injection volume was used. Data were analyzed with the Compass Data Analysis software package (Bruker Corporation).

Turner M.W., Rossi M., Campfield V., French J., Hunt E., Wade E, & McDougal O.M. (2019). Steroidal Alkaloid Variation in Veratrum californicum as Determined by Modern Methods of Analytical Analysis. Fitoterapia, 137, 104281.

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Peptide Purification by hpRP Chromatography

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The labeled peptides were firstly subjected to Sep-Pak SPE cartridges (Waters) to remove salt ions. The hpRP chromatography was performed with Dionex UltiMate 3000 model on an Xterra MS C18 column (3.5 um, 2.1 × 150 mm, Waters). Then the sample were dissolved in buffer A (20 mM ammonium formate, pH 9.5) and eluted with a gradient of 10 to 45% buffer B (80% acetonitrile (ACN)/20% 20 mM NH₄HCO₂) in 30 min, followed by 45% to 90% buffer B in 10 min, and a 5-min hold at 90% buffer B. Forty-eight fractions collected at 1 min intervals were merged into 12 fractions.

Amigo N., Zhang Q., Amadio A., Zhang Q., Silva W.M., Cui B., Chen Z., Larzabal M., Bei J, & Cataldi A. (2016). Overexpressed Proteins in Hypervirulent Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 Compared to E. coli O157:H7 EDL933 Clade 3 Strain. PLoS ONE, 11(11), e0166883.

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Quantification of EVG in PLGA-EVG Nanoparticles

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EVG or EVG in PLGA-EVG nanoparticles (drug encapsulation) was quantified using the LC-MS/MS method as described [17] (link). The LC-MS/MS system used was a liquid chromatography system (Shimadzu Corp., Kyoto, Japan) coupled to a tandem mass spectrometer (AB SCIEX, Triple Quad 5500, Framingham, MA). EVG standards and experimental samples were extracted by adding 3-volumes of cold acetonitrile, which contained 138 nM ritonavir (RTV) as an internal standard (IS). A 6 µL aliquot of extracted samples was injected into an Xterra^® MS C18 column (125 Å, 3.5 µm, 4.6 mm × 50 mm; Waters, Milford) for separation. The mobile phase was consisted of (A) Water with 0.1% formic acid and (B) acetonitrile with 0.1% formic acid (v/v) at a flow rate of 1 mL/min. The gradient elution was as follows: 0–1.5 min, 50% B; 1.5–5.1 min, 60% B (v/v).

Gong Y., Chowdhury P., Midde N.M., Rahman M.A., Yallapu M.M, & Kumar S. (2017). Novel elvitegravir nanoformulation approach to suppress the viral load in HIV-infected macrophages. Biochemistry and Biophysics Reports, 12, 214-219.

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HPLC Analysis of Compounds

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Waters e2695 HPLC system (Waters, Milford, USA) was used for the chromatographic analysis. The analytical column was an XTERRA MS C18 column (4.6 mm × 250 mm, 5 µm) (Waters, Milford, USA). The mobile phase was 0.1% formic acid solution (A)—acetonitrile (B) with gradient elution (0–10 min, 10–25% B; 10–37 min, 10–25% B; 37–41 min, 35–100% B; 41–45 min, 100–10% B). The flow rate was 1.0 mL/min and column temperature was maintained at 25 °C. The detection wavelength was set at 360 nm. The injection volume was 10 μL with needle wash.

Zhang W., Yin Z., Guo Q., Chen L, & Zhang J. (2023). Ionic liquid-based ultrasonic-assisted extraction coupled with HPLC to analyze isoquercitrin, trifolin and afzelin in Amygdalus persica L. flowers. BMC Chemistry, 17(1), 102.

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HPLC Determination of Compound X

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HPLC condition The chromatographic separation was carried out on a Waters X-Terra MS C₁₈ column (250 mm×4.6 mm, 5 µm), operated at 35°. The analytical condition was set as follows, gradient elution by the mixture of mobile phases A (0.05% triethylamine aqueous solution) and B (methanol) at 0-18 min with the ratio of 80-52% A and 20-48% B; at 18-35 min with the ratio of 52-48% A and 48-52% B; and at 35-40 min with the ratio of 48-35% A and 52-65% B and 40-70 min with the ratio of 35-32% A and 65-68% B. The flow rate was 0.8 ml/min and the detection wavelength was set at 284 nm with the sample injection volume of 20 ml.

Liu L.J, & Liu J.N. (2016). A Strategy for Quality Control of Menispermum dauricum DC Based on Cytotoxic Activity and HPLC Fingerprint Analysis. Indian Journal of Pharmaceutical Sciences, 78(1), 143-150.

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HPLC Analysis of Amide Formation

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To confirm amide formation, the supernatant was analysed by high performance liquid chromatography (HPLC) equipped with an XTerra MS C18 column (Waters, Milford, MA) kept at 40 °C. The separation was carried out using a linear gradient of methanol (0–100%) in 0.1% (v/v) formic acid solvent for 15 min at a flow rate of 1.0 mL/min, and detection was performed at a wavelength of 214 nm. Whenever it was necessary, the reactants were collected by an HPLC system equipped with a fraction collector (model CHF122SB, Advantec, Tokyo, Japan). ATP and AMP were measured as reported previously^{35 (link)}.

Hara R., Hirai K., Suzuki S, & Kino K. (2018). A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanism. Scientific Reports, 8, 2950.

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Quantitative Analysis by LC-MS/MS

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Analyses were carried out by using the Prominence LC-20AB/SPD-20A liquid chromatography system that consisted of a LC-20AB binary pump equipped with an online degasser and an autosampler (Shimadzu Co., Kyoto, Japan). The chromatographic system was operated by using the Analyst software 1.5.1 (AB Sciex, Ltd., Framingham, MA, USA). Separations were conducted by using a 50 mm × 2.1 mm I.D. XTerra MS C18 column (Waters Co., Milford, MA, USA).
Detection was performed with an API3200 quadrupole mass spectrometry (AB Sciex, Ltd., Framingham, MA, USA), equipped with turbo ion spray interface, operated in the positive mode, and configured in multiple reaction monitoring mode. The mass spectrometry system was operated by using the Analyst software 1.5.1 (AB Sciex, Ltd., Framingham, MA, USA).

Ogawa-Morita T., Sano Y., Okano T., Fujii H., Tahara M., Yamaguchi M, & Minami H. (2017). Validation of a Liquid Chromatography-Tandem Mass Spectrometric Assay for Quantitative Analysis of Lenvatinib in Human Plasma. International Journal of Analytical Chemistry, 2017, 2341876.

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