LPS, Apelin-13, F13A (a selective APJ receptor antagonist), DPI (an NAPDH oxidase inhibitor, diphenylene iodonium), NAC (a superoxide inhibitor, N-Acetyl Cysteine) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Mouse tumor necrosis factor (TNF)-α, IL-1β, IL-6 and Apelin-13 enzyme linked immunosorbent assay (ELISA) kits were purchased from Cusabio (Houston, TX, USA). The reactive oxygen species assay kit (DCF-DA) was from Applygen (Beijing, China). The H2O2 assay kit was from Beyotime (Shanghai, China). NOX4- small-interfering RNA (siRNA) and PFKFB3 siRNA were provided by GenePharma (Shang hai, China). Mouse PFKFB3 (NM_001177756) overexpressed plasmid was purchased from MiaoLing Plasmid Sharing Platform (Hubei, China). Other reagents are described below.
Pfkfb3 sirna
Manufactured by GenePharma
Sourced in China
PFKFB3 siRNA is a laboratory product designed for the silencing of the PFKFB3 gene, which encodes an enzyme involved in the regulation of glycolysis. This product can be used as a tool for investigating the role of PFKFB3 in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Apelin 13, by Merck Group (1 mentions)
Apelin 13, by Cusabio (1 mentions)
Enzyme linked immunosorbent assay elisa kit, by Cusabio (1 mentions)
H2o2 assay kit, by Beyotime (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Il 1β, by Cusabio (1 mentions)
Interleukin 6 (il 6), by Cusabio (1 mentions)
Nc sirna, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Hiperfect transfection reagent, by Qiagen (1 mentions)
Scrambled sirna, by GenePharma (1 mentions)
3 protocols using pfkfb3 sirna
1
Apelin-13 Modulates Inflammatory Response
2
Overexpression and Silencing of PFKFB3 in Mouse Lung Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Plasmid vector encoding PCBP3 and the empty vector were purchased from Hanbio (Shanghai, China). Primary mouse lung fibroblasts were cultured in six well plates (105 cells/well) and added with 2.5 μg of target plasmid per well. After 12 h, the transfection medium was changed to normal medium. Effects of overexpression on mRNA and protein levels were examined 36 h later. The siRNA targeting mouse PFKFB3 (PFKFB3 siRNA: 5′- CCUCUUGACCCUGAUAAAUTT-3′) were synthesized by Genepharma Co. (Shanghai, China). Primary mouse lung fibroblasts were cultured in six well plates (105 cells/well) and transfected using Lipofectamine 3,000 (Invitrogen, CA) with PFKFB3 siRNA or negative control siRNA (NC siRNA) for 48 h following the manufacturer’s instructions.
3
Silencing PFKFB3 in Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PFKFB3 siRNA and scrambled siRNA were synthesized by GenePharma Co., Ltd. (GenePharma, Shanghai, China). The previously validated siRNA sequence targeting PFKFB3 was shown below: 5′-AGUUGUAGGAGCUGUACUG-3′. siRNAs (100 nM) were introduced into cultured cells with HiPerfect transfection reagent (Qiagen) after reaching 70% confluence [36 (link)]. The transfected PMSCs were used for endothelial differentiation after 48 h. The silencing effects of siRNAs were confirmed by RT-PCR analysis after 48 h transfection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!