Cells were treated with cadmium (10 μM) or vehicle (referred as untreated (UT) in all figures) for up to 72 h. Cell viability was quantified using MTT assays (Cell Biolabs Inc., San Diego, CA, USA). Apoptosis assays were performed using the Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit-I (BD Pharmingen, San Diego, CA) as described previously [30 (link)]. Colony-formation assays were performed to monitor anchorage-independent growth using the CytoSelect™ 96-Well In Vitro Tumor Sensitivity Assay kit (Cell Biolabs Inc.). RWPE-1 and CTPE cells (5 × 103) were harvested and assayed, as previously described [31 (link)].
Annexin 5 fluorescein isothiocyanate fitc apoptosis kit 1
Manufactured by BD
The Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit-I is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the visualization and measurement of apoptotic cells using flow cytometry or fluorescence microscopy.
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2 protocols using annexin 5 fluorescein isothiocyanate fitc apoptosis kit 1
1
Cadmium Cytotoxicity and Apoptosis Assays
2
Apoptosis Induction in Colon Cancer
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Apoptosis induction in colon cancer cells was analyzed by Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit I (BD Pharmingen). The cells were incubated with various concentrations of crocetin for 24 h. Then cells were subjected to washing two times inice-cold PBS followed by treatment with 500 µl binding buffer. To each of the plate, annexin V-FITC solution (5 µl) and PI (5 µl) was added at 37 °C and incubated for1 h. Flow cytometry was performed for the analysis of induction of in the cell cultures.
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