A portion of LV was fixed in 10% neutral buffered formalin overnight, embedded in paraffin and serially sectioned (4 μm sections for LV and liver, and 3 μm sections for kidney). LV sections were stained with picrosirius red (0.1% w/v) to assess cardiac interstitial collagen content (using polarised microscopy to differentiate type I [orange] and type III [green] collagen), or haemotoxylin and eosin (H&E), to quantify cardiomyocyte width. Sections were imaged (picrosirius red, x200 magnification; H&E, x100 magnification) using an Olympus BX43 microscope and quantified by digital image analysis in ImageJ. For the quantification, 10 cells (H&E) from 10 sections (H&E and picrosirius red) were analysed. Kidney sections were stained with periodic acid Schiff (PAS) for measurement of mesangial expansion as previously described (Watson et al., 2010 (link)). Sections were imaged (x400 magnification) using an Olympus BX43 microscope. For the quantification of the proportional area of staining, 15 glomeruli were analysed using Image-Pro Analyser 7.0 (Media Cybernetics). Liver sections were stained with haematoxylin and eosin as previously described (Ritchie et al., 2012 (link)). Scanned imaging was performed by the Monash University Histology Platform, blinded, and NAFLD activity scoring completed independently by pathologists at WuXi AppTec (Kleiner et al., 2005 (link)).
Bx43 microscope
Manufactured by Olympus
Sourced in Japan, United States, Germany, China, United Kingdom, Spain
The Olympus BX43 is a high-performance optical microscope designed for a wide range of applications. It features a sturdy and durable construction, with a built-in illumination system and a range of objective lenses to accommodate various magnification requirements. The BX43 is equipped with a sturdy, ergonomic design that promotes comfortable operation during extended use.
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Lab products found in correlation
Cellsens dimension software, by Olympus (14 mentions)
Cellsens software, by Olympus (10 mentions)
Ab46545, by Abcam (8 mentions)
Vectastain elite abc kit, by Vector Laboratories (8 mentions)
Dp26 camera, by Olympus (7 mentions)
Dab peroxidase substrate kit, by Vector Laboratories (7 mentions)
Cellsens, by Olympus (5 mentions)
Dp72 camera, by Olympus (5 mentions)
Dp73 camera, by Olympus (5 mentions)
Dp74 digital camera, by Olympus (5 mentions)
Dp72 digital camera, by Olympus (4 mentions)
Cellsens imaging software, by Olympus (4 mentions)
Mayer s hematoxylin, by Merck Group (4 mentions)
Pierce coomassie protein assay kit, by Thermo Fisher Scientific (4 mentions)
Matrigel, by Corning (4 mentions)
Nis elements d software, by Nikon (4 mentions)
Ds fi3 camera, by Nikon (4 mentions)
Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (4 mentions)
Cellsens standard software, by Olympus (4 mentions)
Lsm 710, by Zeiss (4 mentions)
497 protocols using bx43 microscope
1
Histological Analysis of Organ Tissues
2
Histological Evaluation of Liver Tissues
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Livers were removed at euthanasia and divided for fixation in 10% neutral buffered formalin or embedded in optimal cutting temperature media. Formalin fixed samples were submitted to AML labs (Jacksonville, FL). Samples were embedded in paraffin and sectioned at 5 μm onto glass slides, followed by staining with hematoxylin and eosin (H&E). Images were taken on an Olympus BX43 Microscope using an Olympus DP26 camera at total magnification of 400x. For mononuclear cell quantification of H&E stained liver slides (n = 6–8 per experimental group), a single 400x area was counted by a blinded technician.
Livers embedded in optimal cutting temperature media were cut at 5 μm sections in a cryostat. Sections were stained with Oil Red O at the Loyola Histology Core. Assessment of slides was performed by a blinded pathologist. Images were taken on an Olympus BX43 Microscope using an Olympus DP26 camera at total magnification of 400x.
Livers embedded in optimal cutting temperature media were cut at 5 μm sections in a cryostat. Sections were stained with Oil Red O at the Loyola Histology Core. Assessment of slides was performed by a blinded pathologist. Images were taken on an Olympus BX43 Microscope using an Olympus DP26 camera at total magnification of 400x.
3
Histological Analysis of Kidney Damage
4
Histological Analysis of Kidney Damage
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Fresh kidney tissues were fixed in 10% neutral buffered formalin overnight, washed once with PBS, and stored in 70% ethanol at 4°C. The tissues were dehydrated and embedded in paraffin by Research Histology Core Laboratory (MD Anderson Cancer Center) according to standard protocols. Embedded tissues were sectioned at a thickness of 5 μm for hematoxylin & eosin (H&E), 4-HNE and MDA staining. H&E stained kidney sections were analyzed using an Olympus BX43 microscope. Organ damage on the renal cortex was evaluated by the following parameters: tubular dilatation, tubule blush border loss, flattened epithelial cells, and sloughing of cells. The primary antibodies, 4-HNE (ab46545, Abcam, 1:200) or MDA (JAI-MMD-030N, Adipogen, 1:100), were incubated overnight at 4°C. Staining was performed using the Vectastain elite ABC kit and DAB peroxidase substrate kit (Vector laboratories). Images were randomly taken from the renal cortex (10 images per mouse) at 200 X magnification using an Olympus BX43 microscope, and the percentage of 4-HNE positive tubular cells per image was analyzed.
5
Morphological Characterization of Parasitic Worms
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Selected specimens were studied morphologically and characteristics of importance, following publications in Table 2 , were measured using an eyepiece micrometre (BX-43 Olympus Microscope, Olympus Corporation, Japan). All measurements are given in millimetres, unless stated otherwise. The range of measurements are given in the format of length x width mm or specified as length or width only. Drawings were made using BX-43 Olympus Microscope, Olympus Corporation, Japan fitted with a drawing tube. Image capture of specimens was conducted using an Upright Motorized Microscope ECLIPSE Ni-E, Nikon, Japan. Morphological description is provided for Isoparorchis sp. and Euclinostomum sp. Due to the poor quality of larval Eustrongylides specimens, molecular method only was used for identification.
6
Chromosome Preparation from Seed Roots
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To obtain the RTCs, the seeds were soaked in a petri dish with distilled water and filter paper for 24 h, then poured off excess water and put seeds in order. After that, set the dishes at 23 °C Incubator in the dark until the seed had roots at a length of 2–3 cm. Next, the root tips were cut and put in moist cuvettes in a one-to-one relationship and then treated the roots for 2 h with nitrous oxide. Later, the root tips could do the next step or be stored at −20 °C in 70% ethanol. Treating the root tips with cellulase (R-10, Yakult Japan, Tokyo, Japan) and pectinase (Y-23, Yakult Japan, Tokyo, Japan) in a 37 °C constant temperature bath for 57 min, grinding them with a pestle, slides with RTCs split phase could be scrutinized to get the chromosomes with an Olympus BX-43 microscope (Olympus Optical Co., Tokyo, Japan).
7
Quantitative Histological Analysis of Lung Inflammation
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For histopathological analysis, the right superior lobes of the lungs were preserved in 10% neutral buffered formalin overnight and embedded in paraffin. Then, the lungs were sectioned at 4–5 μm and stained with hematoxylin and eosin (H&E) and Ziehl–Neelsen stain. The severity of inflammation in the lungs was evaluated using ImageJ (National Institutes of Health, USA, v1.53). Briefly, for quantitative histopathological analysis of H&E staining, all slides were scanned using an Olympus BX43 microscope with a 4× objective (Olympus Optical Co., Tokyo, Japan), and a pathologist assessed and quantified histopathology in a blinded manner. Each slide image per mouse was evaluated with the ToupTek Toup viewer program (v4.11, Toup View Co., Zhejiang, China) to quantify the total size of the inflamed lesions in each mouse. The measured area was presented as mm2.
8
Histological Analysis of Stored Liver
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The liver was stored in 70 % ethanol until histological analysis after storing at Bouin solution for 24 h. A portion of the stored liver was embedded in paraffin and then cut into 5-μm thick semi-serial histological sections using RM2235 microtome (Microsystems GmbH). Then H&E staining was performed (3) . Representative images were acquired at 20× magnification using an Olympus BX43 microscope (Olympus Optical Co.).
9
Cytogenetic Mapping of An. lesteri Mosquito
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The Wuxi laboratory strain (Jiangsu Institute of Parasitic Diseases, Wuxi, China) and a field strain of An. lesteri collected from Hainan were used in this study. The Wuxi strain of An. lesteri has been maintained in the insectary of the Key Technical Laboratory for Prevention and Control of Parasitic Diseases of the Ministry of Health (MOH) in the Jiangsu Institute of Parasitic Diseases (JIPD), Wuxi, China, for over 30 years. The salivary glands were dissected from early fourth-instar larvae of An. lesteri and were used for obtaining polytene chromosome preparations as previously described [14 (link)]. The quality of polytene chromosomes was examined with an Olympus BX43 phase-contrast microscope (Olympus Corp., Tokyo, Japan). Chromosome preparations with clear banding patterns were placed in liquid nitrogen for several minutes, and then cover slips were removed and slides were dehydrated in 50%, 70%, 90% and 100% ethanol for imaging and in situ hybridization. Chromosomes were imaged with an Olympus BX43 microscope with a DP72 digital camera and CellSens imaging software (Olympus Corp.). The best 50 of approximately 100 images were used to construct a cytogenetic map for An. lesteri, using Adobe Photoshop software as previously described [20 (link)].
10
Senescence Assay of cSM-MSCs in OA
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As senescence is thought to play a significant role in OA, it was hypothesized that cSM-MSCs derived from OA joints might undergo senescence earlier compared to normal cSM-MSCs. Therefore, a senescence assay was performed in which normal (n = 6) and OA (n = 6) cSM-MSCs were seeded at a density of 2,5 × 104 cells/cm2 in duplicate in chamber slides (PEZGS018, Millipore). After 24 h (37 °C, 5% CO2/21% O2), cells were fixed with 4% NBF and stained overnight at 37 °C in the dark with 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal (B4252, Sigma-Aldrich), 1 mg/ml, in a solution of 40 mM citric acid/sodium phosphate, 5 mM potassium hexacyano-ferrate (II) trihydrate, 5 mM potassium hexacyano-ferrate (III), 150 mM sodium chloride, and 2 mM magnesium chloride (MgCl2) in distilled water (pH 6.0)). To visualize nuclei, a counterstain with DAPI (62,248, ThermoFisher) was performed. Sections were mounted with FluorSave (345,789, VWR), and imaged using Olympus BX43 microscope (Olympus, Tokyo, Japan). Analysis of the percentage of senescent cells was performed with ImageJ (version 1.48) using the Senescence Counter [22 (link)], which uses the amount of DAPI positive nuclei to determine the amount of X-gal positive cells.
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