Eca109 (Procell Life Science & Technology Co., Ltd) and Kyse150 (Cell Bank, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai) cells were chosen as the representative cell lines of Human ESCC. The cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc., USA) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., USA) and 1% penicillin-streptomycin antibiotic solution. All cultures were maintained in a humidified incubator with 5% CO2 atmosphere at 37°C. ESCC cells were treated with Baicalein (Selleck, China) or DMSO (Solarbio, China) for 24 hrs with or without pretreated with 100 nM 12(S)-HETE (Cayman Chemical, USA) 4 hrs prior to the inhibitor. Or they were transfected with 12-LOX-specific siRNA (si12-LOX, Stealth RNAi siRNA Card for human Alox12, Catalog #1299003; three 12-LOX-specific 20–25 nt siRNA construct, Invitrogen, USA) and non-targeting siRNA (siNC) using EndoFectinTM–MAX (GeneCopoeia, China) with or without treated with 100 nM 12(S)-HETE/5 ng/mL TGF-β1 (Peprotech, USA) for an additional 24 hrs.
Baicalein
Manufactured by Selleck Chemicals
Sourced in United States
Baicalein is a chemical compound used in laboratory settings. It functions as a bioactive flavonoid that can be utilized for various research and analytical purposes. The core function of Baicalein is to serve as a chemical reagent in experimental studies.
Automatically generated - may contain errors
Lab products found in correlation
Erastin, by Selleck Chemicals (2 mentions)
Myricetin, by Selleck Chemicals (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Eca 109, by Procell (1 mentions)
12 s hete, by Cayman Chemical (1 mentions)
Endofectintm max, by GeneCopoeia (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Hrp conjugated goat anti rabbit and anti mouse igg antibodies, by Cell Signaling Technology (1 mentions)
Bcl 2, by Abcam (1 mentions)
Du145, by National Collection of Authenticated Cell Cultures (1 mentions)
S7242, by Selleck Chemicals (1 mentions)
F5879, by Merck Group (1 mentions)
S2268, by Selleck Chemicals (1 mentions)
Ferrostatin 1 fer 1, by Selleck Chemicals (1 mentions)
Dulbecco s modi ed eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Iron chloride fecl3, by Merck Group (1 mentions)
Lipo 1, by Selleck Chemicals (1 mentions)
6 protocols using baicalein
1
Esophageal Squamous Cell Carcinoma Cell Line Assays
2
Baicalein Induces Apoptosis in PCa Cells
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This study was approved by the Qilu Hospital Committee of Shan Dong University. Androgen-independent PCa cell lines (DU145 and PC-3) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All of the cells were grown in RPMI-1640 medium (Hyclone, Utah, USA) containing 10 % fetal calf serum (Gibco, CA, USA) at 37 °C in a 5 % CO2 incubator. Baicalein was purchased from Selleck Chemicals (Houston, TX, USA) and was dissolved according to the manufacturer’ instructions. Antibodies against phospho-AKT (Ser473), AKT, phospho-mTOR, mTOR and GAPDH, and HRP-conjugated goat anti-rabbit and anti-mouse IgG antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against cleaved PARP, Bax, Bcl-2, and survivin were obtained from Abcam (Abcam, Cambridge, UK).
3
Ferroptosis Induction by Baicalein
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HT22 cells were seeded in 24-well plates at a density of 15% and cultured overnight. The plated cells were pretreated with 32 μM baicalein (S2268, Selleck, USA) for 2 h. Then, medium containing FAC (F5879, Sigma, USA) or erastin (S7242, Selleck, USA) at the final concentration of 300 μM and 500 nM were added and cultured for 36 and 8 h, respectively. Alteration of morphology in treated cells was observed under an inverted microscope.
4
Ferroptosis Pathway Modulation
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Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, USA). Ferric ammonium citrate (FAC) and iron chloride (FeCl3) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Baicalein, erastin, ferrostatin-1 (Fer-1), and liproxstatin-1 (Lipo-1) were purchased form Selleck Chemicals (Houston, TX, USA).
5
Dietary Compounds from Selleckchem in Research
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The following dietary compounds were purchased from Selleckchem: apigenin (Selleckchem, Cat#- S2262), baicalein (Selleckchem, Cat#- S2269), baicalin (Selleckchem, Cat#- S2268), berberine hydrochloride (Selleckchem, Cat#- S2271), Cat#- S2268), caffeic acid (Selleckchem, Cat#- S2277), dihydromyricetin (Selleckchem, Cat#- S2399), emodin (Selleckchem, Cat#- S2295), (−)-epigallacatechin gallate (Selleckchem, Cat#- 2250), gossypol acetate (Selleckchem, Cat#- S2303), hematoxylin (Selleckchem, Cat#- S2384), indirubin (Selleckchem, Cat#- S2386), kaempferol (Selleckchem, Cat#- S2314), luteolin (Selleckchem, Cat#- S2320), morin hydrate (Selleckchem, Cat#- S2325), myricetin (Selleckchem, Cat#- S2326), myricitrin (Selleckchem, Cat#- S2327), palmatine chloride (Selleckchem, Cat#- S2397) and quercetin dihydrate (Selleckchem, Cat#- S2347).
6
Sourcing and Storage of Inhibitory Compounds
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Chemical inhibitors were obtained from commercial sources: SSYA10-001 (Glixx Lab #GLXC-20411), Cangrelor (Sigma-Aldrich, #SML2004), Cepharanthine (Selleck, #S4238), Lumacaftor (Selleck, #S1565), Ribavirin (Sigma-Aldrich, #R9644), Baicalein (Selleck, #2268), Myricetin (Selleck, #S2326), Punicalagin (Selleck, #S9131) Scutarellein (Selleck, #S9120), and Tideglusib (Selleck, #S2823). Inhibitors were suspended in the appropriate solvent and stored as per manufacturer's recommendations.
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