Malondialdehyde assay kit

Manufactured by Solarbio

Sourced in China

The Malondialdehyde assay kit is a laboratory reagent used to quantify the levels of malondialdehyde, a biomarker for oxidative stress. It provides a colorimetric method for the detection and measurement of malondialdehyde in biological samples.

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6 protocols using malondialdehyde assay kit

Antioxidant Enzyme Activities and MDA Levels

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The activities of antioxidant enzymes, i.e., superoxide dismutase (SOD) and peroxidase (POD), and contents of the organic compound malondialdehyde (MDA) were determined in L0 and L12 under non-fumigated and S-fumigated treatments, respectively. The activities of SOD and POD were determined using a superoxide dismutase assay kit (Solarbio, Beijing, China) and a peroxidase assay kit (Solarbio, Beijing, China), respectively. The malondialdehyde assay kit (Solarbio, Beijing, China) measured the MDA contents. In brief, collected leaves were ground using liquid nitrogen, and SOD, POD, and MDA were extracted using the extraction buffer. The extracts were centrifuged at 12,000 × g for 10 min, and the supernatants were then measured for SOD, POD activities, and MDA contents using the multimode microplate reader (Tecan, Switzerland) (Yin et al., 2018 (link)). Each indicator had three replicate measurements.

Liu J., Gao Y., Gong F., Hou F., Zhang Z., Cheng X., Du W., Zhang L., Wang J., Xu J., Xing G., Kang X, & Li S. (2021). The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.). Frontiers in Plant Science, 12, 778956.

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Quantifying Plant Stress Responses

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The hyphal fragments were placed on plant leaves. Photographs were taken 36 h after inoculation. The plants transformed into pLGNL were used as controls. Malondialdehyde (MDA) content and catalase (CAT) activity were determined using a Malondialdehyde Assay Kit (Solarbio, Beijing, China) and a Catalase Assay Kit (Solarbio, Beijing, China) according to the manufacturer’s instructions. All treatments were repeated three times. The content of superoxide radical (O₂⁻) and hydrogen peroxide (H₂O₂) in leaves was determined by nitroblue tetrazole (NBT) and 3,3′-diaminobenzidine (DAB) staining as described previously [37 (link),38 (link)].

Wang D., Gong N., Liu C., Li S., Guo Z., Wang G., Shang Q., Wang D., Ji X, & Xin Y. (2022). MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis). International Journal of Molecular Sciences, 23(21), 13372.

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Evaluating Cardiomyocyte Stress Markers

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After cardiomyocyte H/R model establishment, the expression level of LDH was detected with LDH cytotoxicity assay kit (Beyotime, Shanghai, China). The expression level of SOD was detected with total superoxide dismutase assay kit (Solarbio, Beijing, China), and the expression level of MDA was detected with malondialdehyde assay kit (Solarbio). The level of MDA was taken as the disparity of the absorbance at 532 nm and 600 nm.

Yu S., Dong B., Fang Z., Hu X., Tang L, & Zhou S. (2018). Knockdown of lncRNA AK139328 alleviates myocardial ischaemia/reperfusion injury in diabetic mice via modulating miR‐204‐3p and inhibiting autophagy. Journal of Cellular and Molecular Medicine, 22(10), 4886-4898.

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Serum Biomarkers for Myocardial Injury

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Rat blood samples were kept at room temperature for 30 min until the serum precipitated and then centrifuged at 3500 g/min for 10 min in a low-temperature centrifuge for serum extraction. Cardiac troponin I (c Tn I) was measured using a cardiac troponin I kit (Solarbio, China). Lactate dehydrogenase (LDH) was measured using a lactate dehydrogenase kit (Solarbio, China) according to the manufacturer's instructions specified in the kit. Malondialdehyde (MDA) was measured in the myocardial homogenate supernatant using a malondialdehyde assay kit (Solarbio, China).

Wang C.Z., Guo H.Z., Leng J.Z., Liang Z.D., Wang J.T., Luo L.J., Wang S.Q, & Yuan Y. (2024). Exercise preconditioning inhibits doxorubicin-induced cardiotoxicity via YAP/STAT3 signaling. Heliyon, 10(6), e27035.

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Quantifying Oxidative Stress in Fusarium

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The Malondialdehyde Assay Kit (Solarbio, China) was used to determine MDA concentration in 0.1 g powders from F. proliferatum using the thiobarbituric acid (TBA) technique. SpectraMax i3x determination of absorbance and calculation of MDA content according to the instructions were carried out. On a fresh weight basis, the MDA content was given as μmol g⁻¹. Hydrogen Peroxide Assay Kit (Solarbio, China) was used to evaluate the H₂O₂ concentration in 0.1 g powders from F. proliferatum. Finally, the H₂O₂ content was determined using SpectraMax i3x according to the instructions. On a fresh weight basis, the H₂O₂ content was represented as μmol g⁻¹.

Yang J., Xia X., Guo M., Zhong L., Zhang X., Duan X., Liu J, & Huang R. (2022). 2-Methoxy-1,4-naphthoquinone regulated molecular alternation of Fusarium proliferatum revealed by high-dimensional biological data. RSC Advances, 12(24), 15133-15144.

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Comprehensive Leaf Biochemical Analysis

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The electrolyte leakage (EL) value of leaves was measured using a digital conductivity meter as described in our previous study [37 (link)]. The contents of malondialdehyde (MDA) were measured using the thiobarbituric acid (TBA) colourimetric method with malondialdehyde assay kit (Solarbio, Beijing, China), and the contents of soluble sugars were detected using the thiobarbituric acid (TBA) colourimetric method with plant soluble sugar content assay kit (Solarbio, Beijing, China). Similarly, chlorophyll content was determined according to the protocol of a chlorophyll assay kit (Solarbio, Beijing, China). Chlorophyll was extracted using an anhydrous ethanol:acetone (1:2) solution, and the content was determined spectrophotometrically at 645 nm (for chlorophyll a) and 663 nm (for chlorophyll b). The soluble protein content of leaves was determined using Coomassie Brilliant Blue G-250 dye according to the Bradford method, with bovine serum albumin being used as the protein standard [39 (link)]. The content of MDA was expressed as micromoles of MDA per gramme fresh weight, whereas the contents of soluble sugars, soluble proteins, and chlorophyll were expressed in terms of milligrammes per gramme fresh weight.

Wang J., Zhang Y., Yan X, & Guo J. (2020). Physiological and transcriptomic analyses of yellow horn (Xanthoceras sorbifolia) provide important insights into salt and saline-alkali stress tolerance. PLoS ONE, 15(12), e0244365.

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