Pf 915275

Glucose and cortisone were purchased from Sigma-Aldrich (Louis, MO). Selective inhibitor of 11β-HSD1 PF-915275 [22 (link)] was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Epidermal growth factor receptor inhibitor Gefitinib [40 (link)–42 (link)] and GR antagonist Mifepristone [43 (link), 44 (link)] were obtained from Sigma. PF-915275, Gefitinib and Mifepristone were dissolved in DMSO for in vitro studies and in CMC-Na (1%) for in vivo experiments. Antibodies for 11βHSD1, GR, α-myosin heavy chain (α-MyHC) and atrial natriuretic peptide (ANP) were purchased from Santa Cruz Biotechnology. Antibodies for extracellular signal-regulated kinase (p-ERK, ERK) and epidermal growth factor receptor (p-EGFR, EGFR) were obtained from Cell Signaling (Danvers, MA). H9c2 embryonic rat heart-derived cardiomyocyte line was obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Cells were cultured in DMEM medium containing 5.5 mmol/L of D-glucose supplemented with 10% FBS, 100 U/mL of penicillin, and 100 mg/mL of streptomycin. For high glucose treatments, cells were cultured in DMEM medium containing 25 mmol/L of glucose (high glucose, HG). In the cortisone-treated group, cells were treated with 0.1 μM of cortisone in DMEM media.