Log In Sign up
The largest database of trusted experimental protocols

Irdye 680lt goat anti mouse

Manufactured by LI COR
Visit Supplier
Sourced in United States

IRDye 680LT goat anti-mouse is a fluorescent secondary antibody designed for use in near-infrared Western blotting and imaging applications. The antibody is conjugated to the IRDye 680LT dye, which has an excitation maximum of 680 nm and an emission maximum of 694 nm.

Automatically generated - may contain errors

Lab products found in correlation

Irdye 800cw goat anti rabbit, by LI COR (32 mentions) Chronolume, by Chrono-Log (6 mentions) Odyssey imaging system, by LI COR (4 mentions) Anti pplcγ2 y1217, by Cell Signaling Technology (4 mentions) Aypgkf, by GenScript (4 mentions) Odyssey fc imaging system, by LI COR (4 mentions) Irdye 800cw goat anti mouse, by LI COR (4 mentions) Irdye 800cw goat anti rabbit antibody, by LI COR (4 mentions) Pvdf membrane, by Merck Group (4 mentions) Odyssey clx, by LI COR (4 mentions) Irdye 800cw donkey anti rabbit igg, by LI COR (3 mentions) Odyssey blocking buffer, by LI COR (3 mentions) Anti psyk y525 526 mouse y519 520, by Cell Signaling Technology (3 mentions) Anti psyk y348 y342 in mice, by Abcam (3 mentions) Irdye 680lt goat anti rabbit, by LI COR (3 mentions) Odyssey infrared imaging system, by LI COR (3 mentions) Odyssey infrared imager, by LI COR (3 mentions) Anti plcγ2, by Santa Cruz Biotechnology (2 mentions) Ibrutinib, by Selleck Chemicals (2 mentions) Anti syk, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

IRDye 680LT (112 citations) IRDye 680LT Goat anti-Mouse IgG (49 citations) IRDye goat anti-mouse (20 citations) Goat anti-mouse 680LT (18 citations) IRDye 680LT Goat anti-Mouse IgG (H + L) (8 citations) Goat anti-mouse IRDye 680LT secondary antibody (8 citations) IRDye® 680LT labeled goat anti-Mouse (926–68020) (3 citations) IRDye 680LT anti-mouse (2 citations) IRDye-labeled 680LT goat anti-mouse IgG antibody (2 citations) IRDye® 680LT Goat anti-Mouse lgG (2 citations)

53 protocols using irdye 680lt goat anti mouse

1

Western Blot Analysis of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples (20 µg) were separated using 10% SDS-PAGE and were transferred to a polyvinylidene difluoride membrane (PVDF, Merck Millipore, USA). Western blot was performed as previously described (Matsukawa et al., 2017). Primary antibodies used were anti-β-III-Tubulin (CST, Japan); anti-myelin basic protein (MBP) (Sigma, Japan); anti-glial fibrillary acidic protein (GFAP) (Sigma, Japan); anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:200, Santa Cruz, Japan); rabbit anti-Phospho-p53 (S15, CST, Japan) and rabbit anti-p53 (1:1000, CST, Japan). Secondary antibodies were IRDye 800CW donkey anti-rabbit IgG (1:1000) or IRDye 680LT goat anti-mouse (1:20000) from LI-COR, Inc., Lincoln, NE, USA. The signal was detected using the Odyssey FC Imaging System (LI-COR, Inc, NE, USA).
Sasaki K., Davies J., Doldán N.G., Arao S., Ferdousi F., Szele F.G, & Isoda H. (2019). 3,4,5-Tricaffeoylquinic acid induces adult neurogenesis and improves deficit of learning and memory in aging model senescence-accelerated prone 8 mice. Aging (Albany NY), 11(2), 401-422.
+ Open protocol
+ Expand
2

Antibody Labeling and Biochemical Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-Kb-SIINFEKL monoclonal antibody 25D-1.16 mAb (12 (link)) was a kind gift of Drs. Jack Bennink and Jonathan Yewdell (NIAID) and was coupled to the fluorescent dye Alexa 647 using protein labeling kits from Molecular probes (Life technologies) following manufacture’s protocol. Rabbit anti-cytoskeletal actin and rabbit anti-Usp14 Abs were from Bethyl laboratories, while goat mAb anti-GFP was from Novus Biologicals. Mouse mAb FK2 for polyubiquitin was from Enzo. IRDye 680LT goat anti- mouse, IRDye 800CW goat anti-rabbit, and IRDye 680LT donkey anti-goat secondary Abs were from LI-COR. MG-132 and emetine were from Calbiochem and Brefeldin A (BFA) was from Millipore. 1-[1-(4-Fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(1-pyrrolidinyl)ethanone (IU1) was from Cayman chemical. Compounds N-(2-(1-(4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-oxoethyl)-N-methyl-2,3-dihydrobenzo[b][1,4]dioxine-6-sulfonamide (hereafter, 1D18) and 1-(1-(3-chloro-4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-(piperidin-1-yl)ethan-1-one (1B10) were from Enamine. Genetest pre-coated parallel artificial membrane permeability assays (PAMPA) plate system was obtained through Corning. BSA was from Amresco and Shield-1 was obtained through Clontech.
Palmer A.L., de Jong A., Leestemaker Y., Geurink P.P., Wijdeven R.H., Ovaa H, & Dolan B.P. (2017). Inhibition of the deubiquitinase Usp14 diminishes direct MHC class I antigen presentation. Journal of immunology (Baltimore, Md. : 1950), 200(3), 928-936.
+ Open protocol
+ Expand
3

Analyzing C-Myc and Cell Cycle Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
P493–6 cells were treated with DMSO, 0.1 μg ml−1 of Tet, 10 μM STR116 or 10 μM STR118 for 48 hours. Harvested cells were lysed in RIPA buffer, and protein concentration was determined using the BCA assay. Samples were loaded at equal protein concentration, separated by SDS-PAGE and transferred to nitrocellulose membranes (Amersham, 10600001). Membranes were incubated with rabbit anti-C-Myc (1:1,000, Cell Signaling Technology, 18583), mouse anti-CCNB1 (1:1,000, Cell Signaling Technology, 4135), rabbit anti-B-actin (1:4,000, Cell Signaling Technology, 4970) and rabbit anti-LDHA (1:4,000, Cell Signaling Technology, 3582). After washing, membranes were stained with IRDye-conjugated secondary antibodies (IRDye 680LT goat anti-mouse 1:10,000, LI-COR, 926–68020, and IRDye 800CW donkey anti-rabbit 1:10,000, LI-COR, 926–32213), and blots were visualized by LI-COR Odyssey imaging system.
Grobben G.J., Peters S.W., Wisselink H.W., Weusthuis R.A., Hoefnagel M.H., Hugenholtz J, & Eggink G. (2001). Spontaneous Formation of a Mannitol-Producing Variant of Leuconostoc pseudomesenteroides Grown in the Presence of Fructose. Applied and Environmental Microbiology, 67(6), 2867-2870.
+ Open protocol
+ Expand
4

Antibodies for Protein Detection Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The rabbit anti-Arf1 (1:10,000) and rabbit anti-Drs2 (1:2000) antibodies have been described previously (Chen et al., 1999 (link)). Mouse anti-GFP (1C9A5, 1:2000) and mouse anti-Myc (9E10, 1:2000) antibodies were purchased from the Vanderbilt Antibody and Protein Repository (Nashville, TN). Mouse anti-FLAG M2 (Sigma-Aldrich Cat# F3165, RRID:AB_259529, 1:5000) and mouse anti-HA 12CA5 (Sigma-Aldrich Cat# 11583816001, RRID:AB_514505,1:2500) were purchased from Sigma-Aldrich. Mouse anti-Ubiquitin Ubi-1 antibody (Millipore Cat# MAB1510, RRID:AB_2180556, 1:1000) was purchased from EMD Millipore (Billerica, MA). Mouse anti-Ubiquitin VU1 (LifeSensors Cat# VU101, 1:1000) was purchased from LifeSensors (Malvern, PA). All secondary antibodies, including IRDye 680LT Goat anti-Mouse (LI-COR Biosciences Cat# 827–11080, RRID:AB_10795014, 1:20,000), IRDye 800CW Goat anti-Mouse (LI-COR Biosciences Cat# 827–08364, RRID:AB_10793856, 1:20,000), and IRDye 680LT Goat anti-Rabbit (LI-COR Biosciences Cat# 827–11081, RRID:AB_10795015, 1:20,000), were purchased from LI-COR Biosciences (Lincoln, NE).
Xu P., Hankins H.M., MacDonald C., Erlinger S.J., Frazier M.N., Diab N.S., Piper R.C., Jackson L.P., MacGurn J.A, & Graham T.R. (2017). COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. eLife, 6, e28342.
+ Open protocol
+ Expand
5

Western Blot Analysis of HA-Tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells (2x105 cells/well, 12 well plates) were grown overnight prior to transfection (Lipofectamine 2000). 24 hrs post-transfection, cells were lysed by addition of 150 μl/well RIPA buffer supplemented with HALT protease cocktail (Pierce) and incubated for 10mins at 4°C prior to sonication, centrifugation (8,000 x g, 10mins) and storage of supernatant. Lysate samples (80 μl) were mixed with 30 μl 4xLDS (Invitrogen) and 10 μl 10xDTT (Invitrogen), heated to 50°C for 10 mins then loaded (25 μl) and analysed using NOVEX 4–12% BIS-Tris gel electrophoresis (Invitrogen). Following transfer onto nitrocellulose, filters were blocked with Odyssey blocking buffer (Li-Cor) for 1 hr at room temp, then incubated with 1/1,000 mouse mAb anti-HA (16B12, Covance) in blocking buffer/0.1% Tween-20 (BT) overnight at 4°C. Filters were washed x4 in TBST ((TBS: 50mM Tris, 150mM NaCl, pH = 7.9) /0.1% Tween-20), then incubated with 1/10,000 IRDye-680LT goat anti-mouse (Li-Cor) in BT (1hr, room temp) prior to washing x4 in TBST. Filters were then viewed on an Odyssey scanner (Li-Cor).
Davis-Poynter N., Yunis J, & Farrell H.E. (2016). The Cytoplasmic C-Tail of the Mouse Cytomegalovirus 7 Transmembrane Receptor Homologue, M78, Regulates Endocytosis of the Receptor and Modulates Virus Replication in Different Cell Types. PLoS ONE, 11(10), e0165066.
+ Open protocol
+ Expand
6

Immunohistochemical Characterization of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used the following antibodies. Vendors, catalog numbers, and dilutions are shown within parenthesis. PSD-95 (Abcam #18258; 1:1000), GluA2 (Invitrogen #32-0300; 1:200), MAP2 (PhosphoSolutions #1100-MAP2; 1:20,000), copGFP (Evrogen #AB513; 1:3,000), synapsin I (Abcam #AB8; 1:1000), GFAP (Millipore #MAB360; 1:1000), Tuj1 (Millipore #AB15708; 1:1000), POD-conjugated DIG (Roche #1207733; 1:1000), Alexa Fluor 488 goat anti-rabbit (Invitrogen #11008; 1:20,000), Alexa Fluor 488 goat anti-mouse (Invitrogen #11001; 1:20,000), Alexa Fluor 555 goat anti-mouse (Invitrogen #A21422; 1:20,000), Alexa Fluor 633 goat anti-chicken (Invitrogen #A21103; 1:20,000), IRDye 680LT goat anti-mouse (Li-Cor #926-68020; 1:20,000), IRDye 800CW goat anti-rabbit (Li-Cor #926-32211; 1:20,000)
Ho V.M., Dallalzadeh L.O., Karathanasis N., Keles M.F., Vangala S., Grogan T., Poirazi P, & Martin K.C. (2014). GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Molecular and cellular neurosciences, 0, 1-12.
+ Open protocol
+ Expand
7

Western Blot Analysis of Protein Abundance

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For western blot analysis, total cell lysates were prepared as previously described [62 (link)]. Nitrocellulose membranes were incubated overnight with primary antibodies at 4 °C, followed by 1 h incubation with Alexa Fluor-labeled secondary antibodies (IRDye 680LT goat-anti mouse or IRDye 800CW goat anti-rabbit antibodies (LI-COR Biosciences)) at room temperature. Fluorescent images were acquired and quantified by LI-COR Odyssey Imaging System. Antibodies used are detailed in Supplementary materials and methods section.
Reyes-Uribe P., Adrianzen-Ruesta M.P., Deng Z., Echevarria-Vargas I., Mender I., Saheb S., Liu Q., Altieri D.C., Murphy M.E., Shay J.W., Lieberman P.M, & Villanueva J. (2018). Exploiting TERT dependency as a therapeutic strategy for NRAS-mutant melanoma. Oncogene, 37(30), 4058-4072.
+ Open protocol
+ Expand
8

Western Blot Analysis of Cell Lysates

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For western blot analysis, total cell lysates were prepared as previously described [62 (link)]. Nitrocellulose membranes were incubated overnight with primary antibodies at 4 °C, followed by 1 h incubation with Alexa Fluor-labeled secondary antibodies (IRDye 680LT goat-anti mouse or IRDye 800CW goat anti-rabbit antibodies (LI-COR Biosciences)) at room temperature. Fluorescent images were acquired and quantified by LI-COR Odyssey Imaging System. Antibodies used are detailed in Supplementary materials and methods section.
Reyes-Uribe P., Adrianzen-Ruesta M.P., Deng Z., Echevarria-Vargas I., Mender I., Saheb S., Liu Q., Altieri D.C., Murphy M.E., Shay J.W., Lieberman P.M, & Villanueva J. (2018). Exploiting TERT dependency as a therapeutic strategy for NRAS-mutant melanoma. Oncogene, 37(30), 4058-4072.
+ Open protocol
+ Expand
9

Antibody and Reagent Sources for Oxidative Stress Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies and reagents were obtained as follows: anti-heme oxygenase-1 (HO-1) polyclonal rabbit IgG (OSA-150, Assay Design, Ann Arbor, MI), anti-NADPH quinone oxidoreductase1 (NQO1) polyclonal rabbit IgG (2618-1, Epitomics, Cambridge, MA), anti-HSP70 monoclonal mouse IgG (200-301-A27, Rockland, Pottstown, PA), IRDye 800CW goat anti-rabbit (green fluorescent; LI-COR, Lincolon, NE, catalogue number 926-32211), and IRDye 680LT goat anti-mouse (red fluorescent; LI-COR, Lincolon, NE, catalogue number 926–68020). Other reagents including dimethylsulfoxide (DMSO), sodium glutamate, fluorescein diacetate (FDA), hydrogen peroxide (HP), tunicamycin (TM), and Hoechst 33 258 stain were obtained from Sigma (St Louis, MO). The chemical synthesis of D1 has been described previously (Satoh et al., 2011 (link)). Synthesis of analogues D1 and D3 are described later.
Satoh T., Stalder R., McKercher S.R., Williamson R.E., Roth G.P, & Lipton S.A. (2015). Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules Is Regulated by Electrochemical Oxidation Potential. ASN NEURO, 7(4), 1759091415593294.
+ Open protocol
+ Expand
10

Platelet Signaling Pathway Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were purchased from Thermo Fisher Scientific unless otherwise stated. Chrono-lume, used for the detection of secreted ATP, was purchased from Chrono-log corporation. Anti-pSyk Y525/526 (mouse Y519/520) and anti-pPLCγ2 Y1217 were purchased from Cell Signaling Technology. Anti-pSyk Y352 (Y346 in mice) and anti-pSyk Y348 (Y342 in mice) were purchased from Abcam. Anti-Syk and anti-PLCγ2 were purchased from Santa Cruz Biotechnology. Ibrutinib was purchased from Selleckchem. Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor. CRP-XL was purchased from Dr Richard Farndale at the University of Cambridge. AYPGKF was purchased from GenScript.
Dangelmaier C.A., Patchin M., Vajipayajula D.N., Vari H.R., Singh P.K., Wright M.N., Kostyak J.C., Tsygankov A.Y, & Kunapuli S.P. (2023). Phosphorylation of spleen tyrosine kinase at Y346 negatively regulates ITAM-mediated signaling and function in platelets. The Journal of Biological Chemistry, 299(7), 104865.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!